Fig. 4

Construction of BREGRS. (A) LASSO regression analysis identified 10 out of the 27 Breg-related genes as significant predictors for OS. (B) Random forest analysis identified 3 out of the 27 Breg-related genes as significant prognostic factors. (C) CD96, OAS1, and CSH1 were determined as significant genes by both LASSO and random forest analyses, and were included in the multivariate Cox regression model. (D) Protein expression levels of CD96, OAS1, and CSH1 were evaluated in B cells with and without BLCA cells’ education. (E) Immunohistochemical staining of CD96, OAS1, and CSH1 in local BLCA samples revealed their association with BLCA TNM stages. (F) Correlation analysis was performed to assess the relationship between protein expressions of CD96, OAS1, and CSH1 and infiltration levels of Bregs in the local cohort. (G) Investigation of the association of CD96, OAS1, and CSH1 with multiple malignant phenotypes at a single-cell-based pan-cancer level. BREGRS, Breg-related score; LASSO, Least Absolute Shrinkage and Selection Operator; *P < 0.05; **P < 0.01; ***P < 0.001