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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Investigating the impact of regulatory B cells and regulatory B cell-related genes on bladder cancer progression and immunotherapeutic sensitivity

Fig. 6

Experimental validation of BREGRS. (A) The mRNA levels of CD96, OAS1, and CSH1 were measured in the local cohort. (B) The expression difference of CD96, OAS1, and CSH1 was compared between cases with TNM stage I-II and those with TNM stage III-IV. (C) The distribution of TNM stages, Bregs’ infiltration proportion, and BREGRS levels were analyzed in the local cohort. (D) BREGRS was found to be associated with advanced TNM stages in the local cohort. (E) A correlation was observed between BREGRS and the infiltration levels of Bregs. (F) An experimental design was implemented to investigate the functions of CSH1 in the expansion of Bregs. (G) Knockdown of CSH1 resulted in suppressed mRNA expressions of CSH1, IL10, PD-L1, TGFβ, CD38, and CD24 in B cells. (H) Knockdown of CSH1 inhibited the protein expressions of CSH1, IL10, CD24, CD38, PDL1, and TGFβ in B cells. (I) A decreased expansion of Bregs was observed in B cells with CSH1 knockdown. *P < 0.05; **P < 0.01; ***P < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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