Fig. 7
BREGRS was found to be associated with the abundance and cytotoxicity functions of CD8 + T cells. (A) BREGRS showed an association with immune component levels, rather than stromal component levels, in the tumor microenvironment of BLCA. (B) The association of BREGRS with immune subtypes of BLCA cases. (C) Differences in immune cell infiltration were observed between subjects with low and high BREGRS. (D, E) The association of BREGRS with immune cell infiltration levels was evaluated using the CIBERSORT-ABS (D) and XCELL (E) algorithms. (F, G) BREGRS exhibited a correlation with the infiltration proportion of CD8 + T cells based on the CIBERSORT-ABS (F) and XCELL (G) algorithms. (H) Meta-analysis was conducted to pool the Spearman correlation coefficient between BREGRS and the infiltration levels of CD8 + T cells. (I) Cases with high BREGRS and low infiltration of CD8 + T cells showed worse overall survival. (J) An overview of the interactions between Bregs and other immune cells. (K) A study design was implemented to investigate the influence of CSH1 expression in B cells on the cytotoxicity functions of CD8 + T cells. (L) Purity of CD8 + T cells isolated from PBMC using magnetic beads was assessed through flow cytometry analysis. (M, N) Co-incubation of B cells with CSH1 knockdown and SW780 cells education promoted the production of IFNγ (M) and TNFα (N) by CD8 + T cells. (O) CSH1 was found to enhance the expression of immunosuppressive molecules in B cells that were educated by BLCA cells. This ultimately resulted in the expansion of Bregs, a reduction in the cytotoxicity of CD8 + T cells, and the progression of BLCA. *P < 0.05; **P < 0.01; ***P < 0.001