Fig. 6

Knockdown of Csmd1 accelerated the rate of gliomagenesis in a mouse model of PDGFB-induced glioma. A Schematic illustration of the RCAS/tv-a system to induce glioma in mice. B Glial tumor formation was faster in RCAS-PDGFB + shCsmd1 transfected mice in comparison to RCAS-PDGFB + shGL2 mice. C The signal intensity of CSMD1 and total P65 immunostaining was detected in the tumor area of RCAS-PDGFB + shGL2- and RCAS-PDGFB + shCsmd1-induced tumors, respectively. The Signal intensities of (D) CSMD1 (purple) and (E) total P65 (green) were detected in individual mice. F Ctrl and CSMD1-overexpressing clones of U-87 cells were inoculated orthotopically as 50,000 cells per mouse. Mice inoculated with CSMD1-overexpressing U-87 cells had increased survival. Long-rank (Mantel-Cox) test was used for the survival curves. An unpaired t-test was used when comparing 2 samples. Scale bar = 50 µm