Fig. 4

PKCα is required for I3A induced B7-H3 protein expression. a mRNA expression of 9 PKC isoforms was measured in LM7 cells by RT-qPCR (N = 3). The mRNA expression levels were normalized by the PKCα mRNA level. b, c After knockdown with control or PKCα siRNA, B7-H3 and PKCα protein levels were determined by western blot and immunofluorescence analysis post-treatment with DMSO or 0.5 µM I3A for 48 h. b Summary B7-H3 and PKCα protein expression determined by western blot after siRNA (left and middle panels; N = 3) and representative western blot (right panel) are shown. c Summary PKCα and B7-H3 protein expression determined by immunofluorescence assay after siRNA (left and middle panels; N = 4) and representative images (right panel) are shown. B7-H3 (extended blue), PKCα (green), and nuclei (red). Scale bar = 100 μm. Data represents mean ± SD (a, b, and c). *p < 0.05, ****p < 0.0001 by two-way ANOVA (b and c)