Fig. 1
ATG7 is overexpressed in MSI-H/dMMR CRC and correlates with poor prognosis. (A) PPI network of autophagy targets generated by STRING. (B) The top ten hub genes were identified by cytoscape. (C) Univariate analysis of genes associated with OS of patients with CRC in the TCGA. (D) Expression levels of ATG7 in different subtypes of colorectal cancer in TCGA. (E) Protein levels of ATG7 in proficient mismatch repair (pMMR) and deficient mismatch repair (dMMR) tumor tissues were detected by western blot. (F) mRNA levels of ATG7 in pMMR and dMMR tumor tissues were detected by qRT-PCR. (G) The correlation of ATG7 expression and MSI score in TCGA COAD/READ cohort. The correlation analysis between ATG7 and MLH1 (H), MSH2 (I), MSH6 (J), PMS2(K) in TCGA COAD/READ cohort. (L-N) Survival analysis showing the relationship between ATG7 level and the OS of patients diagnosed with MSI-H, MSS or MSI-L CRC in TCGA. (O) Kaplan-Meier analysis was performed to assess the prognostic value of ATG7 in MSI-H CRC in Huashan COAD/READ cohort. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001