Fig. 4

ATG7 inhibition restores MHC-I levels in CRC cells. (A) Localization of MHC-I (green) relative to LC3B (red) positive autophagosomes following ATG7 inhibition in LoVo cells. (B) Localization of MHC-I (green) relative to LAMP1 (red) positive lysosomes following ATG7 inhibition in LoVo cells. Effect of targeting ATG7 on HLA-A, B,C levels in LoVo cells (C) and HCT-116 cells (D) by western blot analysis. (E) Treatment of CRC cells with 150 nM Bafilomycin A1 (BafA1), a lysosomal V-ATPase inhibitor, for the indicated time causes an increase in MHC-I. (F) Effect of BafA1 treatment on expression levels of MHC-I molecules