Fig. 5

GPR65 on TAMs is essential for lactate-stimulation to promote glioma cells malignant progression. A RT-PCR and (B) Western blot analysis of GPR65 expression in macrophages transfected with shNC or shGPR65. C-D CCK8 (C) and clone formation assays (D) were performed to assess cell proliferation of U87 and U251 cells upon stimulation with CMs from TAMs transfected with shNC or shGPR65 following lactate stimulation. E–F Wound healing (E) and transwell assays (F) were conducted to evaluate cell migration and invasion of U87 and U251 cells upon stimulation with CMs from TAMs transfected with shNC or shGPR65 following lactate stimulation. G Western blot analysis the protein expression levels of Vimentin, N-cadherin, and E-cadherin in U87 and U251 cells upon stimulation with CMs from TAMs transfected with shNC or shGPR65 following lactate stimulation. Data are presented as the Mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001