Fig. 1

HSPA4 is upregulated in GC tissues via promoter acetylation. A HSPA4 was upregulated in GC tissues compared with normal tissues in TCGA and GTEx datasets (left) and GEO dataset (GSE54129) (right). B HSPA4 was upregulated in GC tissues compared with paired non-tumor tissues collected at SYSUCC. C HSPA4 protein level was determined in GC tumor tissues and paired non-tumor tissues. β-Tubulin was used as a loading control. D The protein level of HSPA4 was analyzed in GC cell lines and pooled non-tumor tissues (10 cases of non-tumor tissues). β-Tubulin was used as a loading control. E High enrichment of H3K27ac signals in the promoter region of HSPA4. F GEPIA analysis showed that HSPA4 expression was positively correlated with histone acetylation signature. G, H The RNA (G) and protein (H) levels of HSPA4 were detected in GC cells treated with acetyltransferase inhibitor C646 or vehicle control. β-Tubulin was used as a loading control. (*, P < 0.05; **, P < 0.01; ***, P < 0.001)