Fig. 2

HSPA4 overexpression promotes GC cell growth. A HSPA4 expression was positively correlated with tumor proliferation signature in TCGA GC dataset. B The protein level of HSPA4 was determined in GC cells with HSPA4 overexpression or knockdown. β-Tubulin was used as a loading control. C The cell proliferation was determined in GC cells with HSPA4 overexpression or knockdown. D Foci formation results of HSPA4 knockdown AGS cells compared with control cells. E Flow cytometry analysis of EdU(+) cells in HSPA4-overexpressing GC cells. F The image, tumor growth curves and weight of xenografts formed in nude mice by injecting MKN45-HSPA4 and vector control cells subcutaneously (n=5). G Representative pictures of HSPA4 staining in xenografts formed by MKN45-HSPA4 and control cells (scale bar=50 µm). H HSPA4 was determined in tumors formed in 2F (V, vector; H, HSPA4). (*,P<0.05; **, P<0.01; ***, P<0.001)