Fig. 5
From: Targeting FTO induces colorectal cancer ferroptotic cell death by decreasing SLC7A11/GPX4 expression
FTO regulates ferroptosis and cell proliferation via SLC7A11/GPX4. (A-B) The MDA concentration or GSH/GSSG ratio were detected using assay kits in FTO knockdown or vector control CRC cells with or without exogenous expression of SLC7A11. (C-D) The MDA concentration or GSH/GSSG ratio were detected using assay kits in FTO knockdown or vector control CRC cells treated with or without exogenous expression of GPX4. (E-F) The cell proliferation was determined in FTO knockdown or vector control CRC cells with or without exogenous expression of SLC7A11 (or GPX4), and then harvested cells for counting cell number at indicated day 1, day 2, day 3, and day 4 to determine the cell proliferation. (G-H) The MDA concentration were detected using MDA assay kits in FTO knockdown or vector control CRC cells with or without knockdown METTL3 (or YTHDF2). (I-J) The GSH/GSSG ratio was detected using GSH/GSSG assay kits in FTO knockdown or vector control CRC cells with or without knockdown METTL3 (or YTHDF2). (All error bars, mean values ± SEM, p values were determined by unpaired two-tailed Student’s t test of n = 3 independent biological experiments. *p < 0.05; **p < 0.01; ***p < 0.001)