Fig. 7
From: Targeting FTO induces colorectal cancer ferroptotic cell death by decreasing SLC7A11/GPX4 expression
Mupirocin enhances the anti-tumor effects of Erastin and RSL3. (A-B) The coefficient of combination index (CI) value were calculating by cell number counting assay in CRC cells. (C) Tumor growth was compared between xenograft nude mice bearing with CRC PDX, which IP injection with Mupirocin alone, Erastin alone, and combination (n = 4). Tumor volume was calculated for each group at the indicated times. (D) All tumors from nude mouse were shown. (E) Tumor mass of xenograft nude mice injected with PDX tumor treated with treated with Mupirocin alone, Erastin alone, and combination (n = 4). (F) Tumor growth was compared between xenograft nude mice injected with PDX tumor treated with treated with Mupirocin alone, RSL3 alone, and combination (n = 4). (G) All tumors from nude mouse were shown. (H) Tumor mass of xenograft nude mice injected with PDX tumor treated with treated with Mupirocin alone, RSL3 alone, and combination (n = 4). (I-J) HE, Ki67, FTO, SLC7A11, GPX4, and 4HNE were analyzed by IHC in a representative PDX xenograft tumor treated with Mupirocin in the absence or presence of Erastin (or RSL3)(scale bar = 50 μm). (All error bars, mean values ± SEM, p values were determined by unpaired two-tailed Student’s t test of n = 3 independent biological experiments. *p < 0.05; **p < 0.01; ***p < 0.001)