Fig. 3

Forced overexpression of KAT2B inhibits CCA cell growth. (A) KAT2B protein in CCA cells (SG231 and HuCCT1) is lower than in noncancerous biliary epithelial cells (H69), as determined by Western blotting analysis. (B) SG231 and HuCCT1 cells were stably transfected with the pMSCV-KAT2B or the empty control vector. Two colonies for each cell line were selected after the cells were screened by puromycin. The level of KAT2B protein was analyzed by Western blotting assay. (C) Forced overexpression of KAT2B inhibited the cell proliferation of SG231 cells (n = 8) and HuCCT1 cells (n = 6), as measured by WST-1 assay. (D) Anchorage-dependent colony formation assay in SG231 and HuCCT1 cells with KAT2B overexpression (n = 6). (E) Anchorage-independent colony formation efficiency in SG231 cells (n = 6) and HuCCT1 cells (n = 6 for vector control and KAT2B#2, n = 5 for KAT2B#1) with KAT2B overexpression. **P < 0.01