Fig. 4

Overexpression of KAT2B suppressed the growth of CCA tumors in vivo. (A) Experimental outline for CCA xenograft in SCID mice. SG231 or HuCCT1 cells with or without KAT2B stable overexpression were mixed with Matrigel and then the cells were injected into the SCID mice subcutaneously. After 7 weeks of injection, the tumors were collected. (B) The growth curves of CCA tumors with or without KAT2B overexpression in SCID mice (n = 7). Tumor size was monitored every week. (C) Images of xenograft tumors recovered from 7 mice in each group. (D) The weight of CCA tumors with or without KAT2B overexpression in SCID mice (n = 7). (E) Immunohistochemistry for Ki67 using CCA xenograft tissues. The bar graphs show Ki67-positive cells in control and KAT2B-overexpressed tissues (n = 5). *P < 0.05, **P < 0.01