Fig. 6

KAT2B overexpression decreases YAP activity via upregulation of NF2. (A) Western blot analysis to detect YAP nuclear accumulation. Nuclear proteins from SG231 and HuCCT1 cells were used for the analysis. Immunoblotting for histone 3 was used as control. Immunoblotting of α-Tubulin was used to exclude possible contamination of the cytoplasmic protein in the nuclear protein. (B) The mRNA levels of YAP target genes were analyzed by quantitative real-time PCR in SG231 and HuCCT1 cells with or without KAT2B overexpression (n = 4). (C) The effect of NF2 siRNA (NF2 siRNA#1 & siRNA#2) on KAT2B-induced downregulation of YAP protein was examined by Western blotting assay. (D) Quantitative real-time PCR was performed to assess the effects of NF2 siRNA (NF2 siRNA#1 & siRNA#2) on KAT2B-induced downregulation of YAP target genes (n = 5). *P < 0.05, **P < 0.01