Fig. 2

Knockdown of PRMT6 Inhibits Migration, Invasion, and EMT in Glioma. A-B: RNA and protein expression of PRMT6 were analyzed in LN229 and U251 cell lines infected with PRMT6 shRNA or scramble shRNA lentivirus. C: Wound healing assay to evaluate the migration of LN229 and U251 cells with or without PRMT6 knockdown. D: Transwell assay assessing migration and invasion of LN229 and U251 cells with or without PRMT6 knockdown. Scale bar = 200 μm. E: Gene Set Enrichment Analysis (GSEA) based on PRMT6 expression in TCGA glioma dataset, suggesting PRMT6 promotes Epithelial-Mesenchymal Transition (EMT). F: In LN229 cells, PRMT6 knockdown leads to morphological changes resembling epithelial cell transformation, with rounder shape and fewer pseudopodia. G: Expression of EMT-related proteins E-cadherin, N-cadherin, and Vimentin in LN229 and U251 cells with or without PRMT6 knockdown