Fig. 5

YTHDF2 Promotes Migration, Invasion, and EMT in Glioma. A: Scratch assay evaluating the migration rate of LN229 and U251 cells with knocked-down or overexpressed YTHDF2. B-C: Transwell assay assessing the migration and invasion of LN229 and U251 cells with YTHDF2 knockdown or overexpression. Scale bar = 200 μm. D: GSEA analysis using glioma datasets from TCGA and CGGA databases, grouped by YTHDF2 expression, indicating YTHDF2’s promotion of Epithelial-Mesenchymal Transition (EMT). E: Morphological changes in LN229 cells resembling epithelial characteristics post YTHDF2 knockdown, including rounder shape and reduced pseudopodia. F: Evaluation of EMT-related proteins E-cadherin, N-cadherin, and Vimentin in LN229 and U251 cells with YTHDF2 knockdown or overexpression