Fig. 2

Knockdown of CEBPD enhances PTX sensitivity of TNBC cells. MDA-MB-231/PTX, MDA-MB-468/PTX, MDA-MB-453/PTX cells were administered lentivirus-carried sh-CEBPD or sh-NC. A CEBPD protein level in cells determined by WB analysis (n = 3); B viability of cells and the IC50 of PTX examined by CCK-8 assay (n = 3). Stably transfected cells were treated with 15 nM PTX for 48 h. C colony formation ability of cells analyzed by colony formation assay (n = 3); D apoptosis of cells determined by flow cytometry (n = 3); E migration and invasion of cells determined by Transwell assay (n = 3). Differences were analyzed by two-way ANOVA with Tukey's post-hoc test (A, C-E). IC50 of PTX to cells was analyzed by nonlinear fitting analysis (B). *p < 0.05