Fig. 2

miR-4732-3p inhibits the proliferation of NSCLC in vitro and in vivo. A qRT-PCR analysis of miR-4732-3p expression in NSCLC cell lines. B qRT-PCR was performed to examine miR-4732-3p expression in NSCLC cells transfected with miR-4732-3p mimics and inhibitors. C-E Colony formation assays (C-D), and CCK8 assays (E) were performed to evaluate the proliferation ability of NSCLC cells transfected with miR-4732-3p mimics and inhibitors. F-G Cell cycle assays were conducted to examine the effect of miR-4732-3p mimics and inhibitors on NSCLC cell cycle progression. H Western blot analysis was used to detect the expression of G2/M phase-related protein in NSCLC cells transfected with miR-4732-3p mimics and inhibitors. I qRT-PCR was performed to assess the expression of miR-4732-3p in H460 cells following infection with lentivirus engineered to overexpress miR-4732-3p (miR-4732-3p OE) and negative control (miR-NC). J The indicated H460 cells (miR-NC and miR-4732-3p OE) were injected into nude mice and xenograft tumors were harvested. Representative images and measurement of tumor weight in xenograft tumors were shown (n = 6). K Measurement and analysis of tumor volume in xenograft tumors with H460 cells stably overexpressing miR-4732-3p and miR-NC. L Histological examination of xenograft tumors by hematoxylin and eosin (HE) staining and Ki-67 expression levels examined by immunohistochemistry (IHC) (Scale bars = 50 μm). M Western blot analysis investigating the impact of miR-4732-3p on G2/M phase-related protein in vivo. Data are shown as the mean ± SD from at least three independent experiments. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001; ns, not significant