Fig. 5

Knockdown of hnRNPK suppresses NSCLC in vitro and in vivo. A-B The proliferation ability of NSCLC cells transfected with hnRNPK siRNAs was evaluated through CCK8 (A) and colony formation assays (B). C H460 cells were infected with lentivirus engineered to knock down hnRNPK (sh-hnRNPK) and negative control (sh-NC). The protein expressions were analyzed by western blot. D The indicated H460 cells (sh-NC and sh-hnRNPK) were injected into nude mice and xenograft tumors were harvested. Representative images and measurement of tumor weight in xenograft tumors were shown (n = 6). E Measurement and analysis of tumor volume in xenograft tumors of sh-NC and sh-hnRNPK groups. F Histological examination of xenograft tumors through HE staining and analysis of Ki-67 expression levels by IHC (Scale bars = 50 μm). G qRT-PCR analysis of miR-4732-3p expression in xenograft tumor tissues with stably hnRNPK knockdown. H qRT-PCR analysis of fucosylated exosomal miR-4732-3p expression in serum from nude mice with stably hnRNPK knockdown. Data are shown as the mean ± SD from at least three independent experiments. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ^****p < 0.0001; ns, not significant