Fig. 5

RAD51 is highly expressed in NPC tissues and positively correlated with the expression of circCDYL2. A. Representative images of H&E staining, in situ hybridization of circCDYL2, and immunohistochemistry of RAD51 protein using the subcutaneous xenograft tumor tissues. 40×, scale bar = 20 μm. B. Statistical results of the relative expression levels of circCDYL2 and RAD51 in the subcutaneous xenograft tumor tissues according to Fig. 5A. C. Correlation between circCDYL2 and RAD51 expression was analyzed in the subcutaneous xenograft tumor tissues according to Fig. 5A. R = 0.8353. D. Representative immunohistochemistry results of RAD51 protein in 102 paraffin-embedded NPC tissues, compared with 28 adjacent nasopharyngeal epitheliums. 20×, scale bar = 50 μm, and 40×, scale bar = 20 μm. E. Overall survival analysis of NPC patients with low and high RAD51 expression using a Kaplan-Meier curve. F. Correlation between circCDYL2 and RAD51 expression was analyzed in 102 nasopharyngeal carcinoma tissues using the Pearson correlation analysis. R = 0.7026. G. The expression of RAD51 was analyzed in NPC and NPE tissues using four NPC gene expression profile data (GSE12452, GSE53819, GSE64634, and GSE61218). Data were represented as mean ± SD. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001