Fig. 11

NPs-mediated upregulation of circPDE5A and PDE5A-500aa inhibits ESCC metastasis in vivo. A Schematic representation of a lung metastasis model treated with NPs. B Bioluminescence images of mice in each group at the end of the experiment (n = 5). C Schematic diagram and HE staining of representative lung tissue from each group. Bar represents 2,000 μm. D Number of metastatic lung nodules in each group (n = 5). E IHC staining for PIK3IP1, PI3K, p-AKT, E-cadherin, and N-cadherin in tumor tissues after systemic treatment in subcutaneous xenograft tumor nude mice with ESCC in each group. Bar represents 50 μm. ***P < 0.005. ns, not significant