Fig. 2
CircPDE5A upregulation inhibits ESCC proliferation and metastasis in vitro and in vivo. A RT-qPCR detection of linear PDE5A transcripts and circPDE5A level in KYSE30 cells after treatment with vector and circPDE5A plasmid. B–D The proliferative capacity of KYSE30 cells assessed by CCK8 (B), plate cloning (C), and EdU (D) assays after treatment with vector and circPDE5A plasmid. Bar in (D) represents 50 μm. E Motility of KYSE30 cells assessed by wound healing assay after treatment with vector and circPDE5A plasmid. Bar represents 100 μm. F Migration and invasion ability of KYSE30 cells assessed by transwell assay after treatment with vector and circPDE5A plasmid. Bar represents 1,000 μm. G WB to detect EMT marker protein levels in KYSE30 cells after treatment with vector and circPDE5A plasmid. H Images of nude mouse xenograft tumors (n = 6) generated by subcutaneous injection of KYSE30 cells transfected with vector and circPDE5A, respectively. I Volume growth curves of nude mouse xenograft tumors. Tumor volume measured every 7 days after inoculation for 6 weeks (n = 6, unit: mm3). J The weight of xenograft tumors in nude mice measured at the end of the experiment (n = 6, unit: mg). K Representative bioluminescent images of lung metastases formed by tail vein injection of KYSE30 cells transfected with vector and circPDE5A, respectively (n = 6). L Diagram and HE staining of representative lung tissue from each group. Bar represents 2,000 μm. M Number of metastatic lung nodules in each group (n = 6). ***P < 0.005. ns, not significant