Figure 1From: Erratum to: renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagyInduction of cell death by EA in A498 RCC cells. A498 cells were treated with EA at 50 and 100 nM. Control cells received 0.1% DMSO (vehicle). All conditions were performed in triplicate. Cells were then incubated with additions for 24 or 48 h before measuring viability using the PrestoBlue® assay (A). A498 cells were treated with 100 nM EA or vehicle for 24 and 45 h durations. Apoptosis was determined by measuring cytoplasmic histone-associated-DNA-fragments using the Cell Death Detection ELISAPLUS assay kit (B). A498 cells were treated with 100 nM EA or with 0.1% DMSO (control) for 24 and 46 h. Cells were then trypsinized, washed with ice cold PBS, and stained with Alexa Fluor® 488 annexin V and PI and analyzed by flow cytometry (C).Back to article page