Open Access

Erratum to: renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagy

  • Richard T Williams1,
  • Alice L Yu1, 2, 3,
  • Mitchell B Diccianni1,
  • Emmanuel A Theodorakis4 and
  • Ayse Batova4Email author
Journal of Experimental & Clinical Cancer Research201433:95

https://doi.org/10.1186/s13046-014-0095-4

Published: 5 December 2014

The original article was published in Journal of Experimental & Clinical Cancer Research 2013 32:57

Abstract

No abstract

Erratum

In the original publication of this manuscript [1], Figure 1 Panel C shows three flow cytometry plots. All values reported in the figure are correct, but a duplicate of the EA plot was mistakenly used for the Vincristine plot. This mistake did not affect our results or discussion as the text refers to the correct experimental values. The corrected Figure 1 is shown below. We apologize for any confusion this may have caused.
Figure 1

Induction of cell death by EA in A498 RCC cells. A498 cells were treated with EA at 50 and 100 nM. Control cells received 0.1% DMSO (vehicle). All conditions were performed in triplicate. Cells were then incubated with additions for 24 or 48 h before measuring viability using the PrestoBlue® assay (A). A498 cells were treated with 100 nM EA or vehicle for 24 and 45 h durations. Apoptosis was determined by measuring cytoplasmic histone-associated-DNA-fragments using the Cell Death Detection ELISAPLUS assay kit (B). A498 cells were treated with 100 nM EA or with 0.1% DMSO (control) for 24 and 46 h. Cells were then trypsinized, washed with ice cold PBS, and stained with Alexa Fluor® 488 annexin V and PI and analyzed by flow cytometry (C).

Notes

Declarations

Authors’ Affiliations

(1)
Department of Pediatrics, Hematology/Oncology, University of California
(2)
The Genomics Research Center, Academia Sinica, University of California
(3)
Stem Cell & Translational Cancer Research Center, Chang Gung Memorial Hospital
(4)
Department of Chemistry and Biochemistry, University of California

References

  1. Williams RT, Yu AL, Diccianni MB, Theodorakis EA, Batova A: Renal cancer-selective Englerin A induces multiple mechanisms of cell death and autophagy. J Exp Clin Cancer Res. 2013, 32: 57-10.1186/1756-9966-32-57.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Williams et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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