Expression of Brachyury is up-regulated in human HCCs. (A) Expression of Brachyury in one human normal liver cell lines (QZG) and four hepatoma cell lines was examined by western blot assay. (B) Brachyury expression in 40 pairs of HCC and the surrounding tissues were detected by real-time PCR. (C) Representative western blot showing the expression of Brachyury protein in tumor tissue (T) and paired peri-tumor tissue (N) from eight HCC patients. (D) Relative immunohistochemical staining of Brachyury expression in paired HCC tissue samples (n = 112), and the representative view (E).