Fig. 4

Dppa4 expression in cell lines and its knockdown inhibits cancer cell proliferation. a Dppa4 protein levels and (b) mRNA levels in 8 colorectal cancer cell lines. c Western blot analysis, and (d) real-time PCR analysis of Dppa4 expression in stable knockdown CaCo2 and HCT8 cell lines. e Effects of Dppa4 knockdown on proliferation was evaluated by Cell Counting Kit-8 assays and (f) plate colony formation assays. g Real-time PCR analysis of proliferation related genes and G1/S transition genes. (* P < 0.05, *** P < 0.01)