Fig. 3

HAT1 transcriptionally increases PD-L1 expression in pancreatic cancer cells. a-c, PANC-1, MIA PaCa-2 and BxPC-3 cells were infected with lentivirus vectors expressing control or HAT1-specific shRNAs. Forty-eight hours postinfection, the cells were harvested for Western blotting (a), RT-qPCR analysis (b) and FACS analysis (c). The data shown are the mean values ± SD from three replicates. *, P < 0.05; **, P < 0.01. d and e, PANC-1 and MIA PaCa-2 cells were transfected with pcDNA3.1, 1 μg Flag-HAT1 or 4μg Flag-HAT1 plasmids. Then, 24 h posttransfection, cells were harvested for Western blotting (d) and RT-qPCR analysis (e). The data shown are the mean values ± SD from three replicates. **, P < 0.01; ***, P < 0.001. f and g, PANC-1 and BxPC-3 cells were treated with serial concentrations of ascorbate for 24 h and cells were harvested for Western blotting (f) and RT-qPCR analysis (g). The data shown are the mean values ± SD from three replicates. **, P < 0.01; ***, P < 0.001.h and i, BxPC-3 cells were infected with the indicated constructs. After 48 h, cells were treated with or without ascorbate for another 24 h and the cells were harvested for Western blotting (h) and RT-qPCR analysis (i). The data shown are the mean values ± SD from three replicates. ns, not significant; **, P < 0.01