Fig. 8

Celastrol suppressed the growth of orthotopic xenografts of U251 cells in vivo. a One week after inoculation, MRI scanning of glioma was performed on a 7.0 T MR scanner. b One week after inoculation, H&E staining of tumor tissue was carried out. Scale bars = 50 μm (c) Tumor volumes determined by MRI at 0, 7 and 14 days after inoculation in the 5 different groups. d Tunel assay and immunohistochemical staining of tumor specimens. Scale bars = 50 μm. e The levels of cleaved caspase-3, LC3B, p-JNK, p-Akt and p-mTOR in tumor tissues were detected by western blotting. β-actin was used as an internal control. Data are presented as the Mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, significantly different compared with the untreated control group