Fig. 4

MEN1 silencing results in nuclear translocation of β-catenin and an increase in its binding to the MYC promoter in AR-independent PCa cells. a Western blot analysis of β-catenin expression in different subcellular fractions in siCtrl or siMEN1(1) + (3)-treated DU145 and PC3 cells as indicated. b Double IF staining showing menin and β-catenin in siCtrl or siMEN1(1) + (3)-treated DU145 cells. Scale bar = 25 μm. ChIP-qPCR analysis assessing β-catenin (c) or menin (d) binding to the MYC promoter and the MYC 3’enhancer in siCtrl or siMEN1(1) + (3)-treated DU145 cells. reChIP analysis evaluating the effect of MEN1-KD (e) and CTNNB1-KD (f) on menin (left panel) and β-catenin (right panel) co-occupancy on the MYC promoter in DU145 cells. Representative blots of three independent experiments