Fig. 7

MI503-treated PC3-GFP xenografts display increased tumor growth with nuclear overexpression of β-catenin. a Representative images of HE stained xenografts treated with DMSO (upper) and MI503 (lower). Scale bar = 200 μm. b qRT-PCR analysis evaluating the number of PC3-GFP cells transplanted in mouse kidney treated (n = 11) or not (n = 9) with MI503. Representative images of IHC staining (c, Scale bar = 100 μm) for menin, JunD and β-catenin or IF staining (d, Scale bar = 50 μm) for JunD and β-catenin in xenografts in the Ctrl group (DMSO treatment, upper panels) or MI503 treatment group (lower panels) as indicated. e Data mining analyses investigating the clinical correlation between MEN1 and CTNNB1 mRNA expression in primary prostate cancer (left panel) and mCRPC (right panel) using existing prostate cancer datasets. f Schematic summary of oncosuppressive functions of menin in AR-independent PCa cells. Representative blots of three independent experiments