Fig. 6

miR-218-5p inhibitor recovered phenotypes of mice induced by Parvimonas micra. A The mimics, inhibitor, or negative control (NC) of miR-218-5p was transfected into LoVo cells, and the MTT assay was used to detect cell proliferation. B The miR-218-5p inhibitor, miR-218-5p inhibitor negative control, or RNase free ddH₂O was added into LoVo cells and then coincubated with Pm-42, and the MTT assay was used to detect cell proliferation. Data are expressed as mean ± SD from 3 independent experiments. C LoVo or HT-29 cells treated with Pm-42, DH5α, or PBS were subcutaneously injected into male BALB/C nude mice to produce xenograft tumors in animals. After nine days of cell injection, the antagomir was injected locally into the tumor every three days for a total of five injections. D–G LoVo cells treated with Pm-42, DH5α, or PBS were subcutaneously injected into male BALB/C nude mice to produce xenograft tumors in animals. Data are expressed as mean ± SD, n = 5. D Images of xenograft mice. E The change of tumor size in xenograft mice. F The weight of tumor in xenograft mice. G The detection of 17 cytokines in the serum of xenograft mice. H Immunohistochemistry was used to detect the PTPRR levels in xenograft mice of LoVo cells. I Western blotting was used to detect Ras, ERK1/2, p-ERK1/2, c-Fos and PTPRR in the xenograft mice of LoVo cells. Data are expressed as mean ± SD from 3 independent experiments