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Correction: Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling
Journal of Experimental & Clinical Cancer Research volume 42, Article number: 70 (2023)
Correction: BMC Plant Biol 37, 207 (2018)
https://doi.org/10.1186/s13046-018-0878-0
Following publication of the original article [1], the author identified an error in the images of Fig. 2 and Fig. 3, specifically:
Furthermore, under Real-time PCR assay (RT-PCR) of Methods section Table 1 citation has to be replaced with Supplementary Table 1. The correct sentence should read as:
‘The sequences of the primers used for the RT-PCR are listed in Supplementary Table 1.’
AS-IV inhibited macrophage M2 polarization. Macrophages derived from THP-1 cells were stimulated with IL-4/IL-13 with or without AS-IV (80 μM) for 48 h. a Flow cytometry was used to quantify the expression of CD206, an M2 macrophage marker, and CD86, an M1 marker. b QT-PCR was performed to detect gene levels in M2 and M1 macrophages. M2-associated genes included PPARγ, Arg-1, and CD206, and M1-associated genes included iNOS, TNF-α, and COX2. c Representative images of M0 and M2 macrophages with and without AS-IV stained with antibodies against Arg-1 (green) and with DAPI (blue). d The levels of IL-10 and TGF-β in the cell culture supernatants were measured by ELISA. Data are presented as the mean ± SEM from three independent experiments. Compared to M0, **p < 0.01, *p < 0.05, and n.s., no significance; compared to M2, ##p < 0.01, #p < 0.05, and n.s., no significance
AS-IV inhibited the migration and invasion of tumor cells. Macrophages derived from THP-1 were stimulated with IL-4/IL-13 with or without AS-IV (80 μM) for 48 h, and the conditioned medium (CM) was collected. A549 and H1299 cells were cultured with different CM. a and b The effect of AS-IV on A549 and H1299 invasion and migration was evaluated by wound scratch assay and trans-well assay, respectively. c and d The mRNA levels of genes associated with migration and angiogenesis in macrophages. Data are presented as the mean ± SEM from three independent experiments. Compared to M0, **p < 0.01, *p < 0.05, and n.s., no significance; compared to M2, ##p < 0.01, #p < 0.05, and n.s., no significance
Reference
Xu F, Cui WQ, Wei Y, et al. Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling. J Exp Clin Cancer Res. 2018;37:207. https://doi.org/10.1186/s13046-018-0878-0.
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Supplementary Information
Additional file 1: Supplementary Table 1.
Sequences of the primers used for PCR.
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Xu, F., Cui, WQ., Wei, Y. et al. Correction: Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling. J Exp Clin Cancer Res 42, 70 (2023). https://doi.org/10.1186/s13046-023-02643-y
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DOI: https://doi.org/10.1186/s13046-023-02643-y