Fig. 2

The expression of EGFR regulates the proliferation of PRV-LAV. A, B GBM cells and PK-15 cells were pretreated with vehicle or 5 μM kinase inhibitor and were then infected with PRV-LAV-mNeonGreen (MOI = 0.001). The inhibition rates against PRV infection in GBM and PK-15 cells were calculated by Harmony imaging and analysis software. The results are presented on a scatter plot. Each point on the plot represents a kinase inhibitor (A). Kinase inhibitors with inhibition rates of at least 80% in both GBM and PK-15 cells were classifed according to their molecular function in signaling pathways (B). This experiment was repeated three times. C The expression of PRV gB was analyzed by western blotting after cancer cells were pretreated with the EGFR inhibitor afatinib and then infected with PRV-LAV HB2000 (GBM, MOI = 0.001; HepG2 and Panc-1, MOI = 0.01) and cultured for 48 h. D The expression of EGFR in 293FT and EGFR-OE 293FT cells was analyzed by western blotting. E, F 293FT and EGFR-OE 293FT cells were infected with PRV-LAV-mNeonGreen (MOI = 0.1, 1). Phase-contrast and fuorescence micrographs were acquired with an Opera Phenix High Content Screening System (E), with cell viability assays were performed (F) 24 h and 48 h post-infection. E Scale bars, 100 μm. F Data are presented as the mean ± s.d. values (n = 6). 293FT cells vs. EGFR-OE 293FT cells. A t test was used to determine the signifcance of diferences in the percentages of viable cells post-viral infection. G-I Knockdown of EGFR expression in HepG2 cells suppressed the proliferation of PRV-LAV. The expression of EGFR in HepG2 and KD-EGFR HepG2 cells was analyzed by western blotting (G). HepG2 and KD-EGFR HepG2 cells were infected with PRV-LAV-mNeonGreen (MOI = 0.1, 1). Phase-contrast and fuorescence micrographs were acquired with an Opera Phenix High Content Screening System (H), with cell viability assays were performed (I) 24 h and 48 h post-infection. Scale bars, 100 μm. The data are presented as the mean ± s.d. values. Data are presented as the mean ± s.d. values (n = 6). The black bars indicate the mean values. A t test was used to determine the signifcance of diferences in the percentages of viable cells post-viral infection