- Open Access
Establishment of a new human osteosarcoma cell line, UTOS-1: cytogenetic characterization by array comparative genomic hybridization
© Yasuda et al; licensee BioMed Central Ltd. 2009
- Received: 07 January 2009
- Accepted: 25 February 2009
- Published: 25 February 2009
The cytogenetic characteristics of osteosarcoma (OS) remain controversial. The establishment of a new human OS cell line may improve the characterization. We report the establishment of a new human osteosarcoma cell line, UTOS-1, from a typical osteoblastic OS of an 18-year-old man. Cultured UTOS-1 cells are spindle-shaped, and have been maintained in vitro for over 50 passages in more than 2 years. Xenografted UTOS-1 cells exhibit features typical of OS, such as production of osteoid or immature bone matrix, and proliferation potency in vivo. UTOS-1 also exhibit morphological and immunohistochemical characteristics typical of osteoblastic OS. Chromosomal analysis by G-band show 73~85 chromosomes with complicated translocations. Array CGH show frequent gains at locus DAB2 at chromosome 5q13, CCND2 at 12p13, MDM2 at 12q14.3-q15, FLI and TOP3A at 17p11.2-p12 and OCRL1 at Xq25, and show frequent losses at HTR1B at 6q13, D6S268 at 6q16.3-q21, SHGC17327 at 18ptel, and STK6 at 20q13.2-q13.3. The UTOS-1 cell line may prove useful for biologic and molecular pathogenetic investigations of human OS.
- Array Comparative Genomic Hybridization
- Human Osteosarcoma Cell Line
- Numerical Chromosome Abnormality
- Locus DAB2
- GenoSensor Array
Osteosarcoma (OS) is the most common malignant bone tumor in adolescents and young adults, and is characterized by proliferation of tumor cells which produce osteoid or immature bone matrix. Despite recent advances in multimodality treatment consisting of aggressive adjuvant chemotherapy and wide local excision, pulmonary metastasis occurs in approximately 40 to 50% of patients with OS and remains a major cause of fatal outcome [1–3].
There have been several reports describing xenotransplantation models of human OS [4–7], but characterization of human OS at the molecular cytogenetic level has been limited [8, 9]. We describe the establishment and characterization of a new human OS cell line, designated as UTOS-1, derived from a conventional osteoblastic OS. In addition, we analyze chromosomal aberrations and DNA copy number changes in UTOS-1 by array comparative genomic hybridization (aCGH).
Source of Tumor Cells
Tumorigenicity in severe combined immunodeficiency (SCID) mice
To determine the tumorigenicity of the UTOS-1 cell line in vivo, 1 × 108 cells were washed, suspended in phosphate-buffered saline (PBS), and injected subcutaneously into the leg of 4-week-old male SCID mice (CB-17/Icrscid; Clea Japan Incorporation, Osaka, Japan). Also, tumor growth in vivo was measured by calculating tumor volume based on the measurement of 2 perpendicular diameters using a caliper . The volume was estimated using the following formula: 0.5 × L × (S)2, where L and S are the largest and smallest perpendicular tumor diameters, respectively.
Establishment of the tumor cell line
Tumor cells were seeded in a 25 cm2 plastic flask (35–3109; Falcon, Franklin Lakes, NJ, USA) . These cells were cultured in RPMI 1640 (MP Biomedicals, Solon, OH, USA), supplemented with 100 mg/ml streptomycin (Meiji Seika, Tokyo, Japan), 100 U/ml penicillin (Meiji Seika) and 10% fetal bovine serum (FBS; Funakoshi, Tokyo, Japan), at 37°C in a humidified atmosphere of 5% CO2 and 95% air. The medium was replaced once per week. When semiconfluent layers were obtained, the cells were dispersed with Ca2+- and Mg2+-free PBS containing 0.1% trypsin and 0.02% EDTA solution, and were then seeded in new flasks for passage. The configuration of tumor cells was almost equalized after the 3rd generation. These procedures were serially performed until the UTOS-1 cell line was established.
Cell growth in vitro
To determine the doubling time, UTOS-1 cells were seeded in each well of 96-well dishes (Corning Costar, Tokyo, Japan) with fresh medium containing 100 μl of RPMI 1640 with 10% FBS. Cell growth was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Cell Counting Kit-8, Dojindo, Tokyo, Japan) . A volume of 10 μl of MTT was added to each well, followed by mixing. Plates were incubated for 3 hours at 37°C in a humidified atmosphere of 5% CO2 and 95% air. Formazan levels, which correspond to the number of viable cells, were quantified using a microplate reader (model 450; Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 450 nm. The absorbance of each well was evaluated at 6, 12, 24, 48, 72, 96 and 120 hours after seeding. Triplicate wells were used for each observation.
Cells were cultured in chamber slides (Lab-Tek; Nalge Nunc International, Naperville, IL, USA). For the detection of mesenchymal phenotype, we used 3 monoclonal antibodies: anti-AE1/AE3, anti-keratin mix, and anti-vimentin. Also, to assess osteoblastic differentiation, we used 2 monoclonal antibodies: anti-OP and anti-OC. ALP activity of UTOS-1 cells was estimated using a modified version of a cytochemical method described elsewhere , with naphthol AS-MX phosphate-fast blue RR staining (ALP staining kit; Muto Pure Chemicals Corporation, Tokyo, Japan).
Cells grown in chamber slides were washed in PBS, fixed in 4% paraformaldehyde for 15 minutes at room temperature, and then fixed in methanol for 20 minutes at -20°C. The cells were incubated with each of the primary antibodies for 24 hours at 4°C. Immunoreaction products were detected using DAKO envision (DAKO Sytomation, Carpinteria, CA, USA), and were visualized after adding diaminobenzidine (DAB; DAKO) as the chromogen.
RNA extraction and reverse-transcription polymerase chain reaction (RT-PCR)
The oligonucleotide primer sequences and PCR conditions for ALP, OP, and OC in this study.
(5' to 3')
(temperature, cycle number)
For cytogenetic analysis, preparations of metaphase chromosomes from UTOS-1 cells at passage 15 were obtained, and were banded with Giemsa-trypsin . Karyotypes were described using the short version of the International System for Human Cytogenetic Nomenclature .
DNA extraction and array CGH
Genomic DNA was extracted from UTOS-1 cells at passage 15. The CGH procedure used was similar to published standard protocols . Genomic DNA was isolated from tumor samples using standard procedures including proteinase K digestion and phenol-chloroform extraction.
Array CGH was performed using the GenoSensor Array 300 system, following the manufacturer's instructions (Vysis, Downers Grove, IL, USA). This array contains the 287 chromosomal regions that are commonly altered in human cancer, such as telomeres, regions involved in microdeletions, oncogenes, and tumor suppressor genes. Tumor DNA (100 ng) was labeled by random priming with fluorolink cy3-dUTP, and normal reference (control) DNA was labeled using the same method with cy5-dUTP. The tumor and control DNAs were then mixed with Cot-1 DNA (GIBCO-BRL, Gaithersburg, MD, USA), precipitated, and resuspended in microarray hybridization buffer containing 50% formamide. The hybridization solution was heated to 80°C for 10 minutes to denature the DNA, and was then incubated for 1 hour at 37°C. Hybridization was performed for 72 hours in a moist chamber, followed by a post-hybridization wash in 50% formamide/2 × SCC at 45°C. Slides were mounted in phosphate buffer containing 4',6-diamidino-2-phenylindole (DAPI; Sigma, St. Louis, MO, USA). Fluorescence intensity images were obtained using the GenoSensor Reader System (Vysis) according to the manufacturer's instructions. For each spot, the total intensity of each of the 2 dyes and the ratio of their intensities were automatically calculated. The diagnostic cut-off levels representing gains and losses were set at 1.2 (upper threshold) and 0.8 (lower threshold). This assay was performed in triplicate, and common aberrations were considered to be meaningful aberrations.
Tumor growth in vivo
Cell growth and morphological findings in vitro
Immunohistochemical and cytochemical findings
There have been several reports describing xenotransplantation models of human OS [4–7]. In the present study, the parent tumor, the cultured tumor cells, and the xenografted tumor exhibited features typical of OS, as reported previously [15, 17]. Cultured UTOS-1 cells have a spindle shape with several nucleoli, which is similar to the original tumor cells. Biochemical characteristics of UTOS-1, such as cell growth rate and osteoblastic activity, have not changed during the 2 years that they have been maintained. Immunohistochemically, the UTOS-1 cells remain positive for ALP, OP and OC. After implantation from cell culture into SCID mice, UTOS-1 cells grew in vivo, producing osteoid resembling that of the original tumor. Abundant osteoid tissue formed in the xenografted tumors and reimplanted tumors. These findings suggest that UTOS-1 cells have an osteoblastic phenotype and retain the characteristics of the original tumor. The population-doubling time of UTOS-1 cells in vitro is 40 hours, which is similar to that of other OS cell lines [4, 6, 18].
Several reports indicate that OS cells have karyotypes with multiple numerical rearrangements and complex structural rearrangements [9, 19–21]. Together, the results of several cytogenetic surveys indicate that OS cells frequently have structural alterations at chromosome bands 1p11-13, 1q11-12, 1q21-22, 11p15, 12p13, 17p11-3, 19q13, and 22q11-13, and frequently have the numerical chromosome abnormalities +1, -9, -10, -13, and -17. In UTOS-1 cells, the clonal chromosomal abnormalities that were detected were triploidies. The chromosomal rearrangements that were observed in UTOS-1 involved chromosomes 1q11-12, 11p15, 19q13, and 22q11-13. The numerical chromosome abnormalities that were observed in UTOS-1 included +1, -9, -10, -13, and -17. These findings are similar to studies of other OS cell lines .
Metaphase CGH studies of OS have identified frequent gains at chromosome bands 1p32, 1q21, 5p13, 6p12, 8q24, 8cen-q13, 17p11.2, and Xp21, and frequent losses at bands 6q16, 10p12pter, and 10q22-q26 [22, 23]. Recent metaphase CGH studies of OS have focused on amplifications at chromosomes 8q, 6p, and 17p [22, 24]. Advances in mapping resolution of microarray CGH [25, 26] have greatly improved its resolving power, such that it now provides greater detail than metaphase CGH regarding the complexity and exact location of genomic rearrangements leading to copy number imbalances.
In the present study, chromosome 12 showed several distinct regions of focal amplification, occurring at gains of CCND2 at 12p13 12q13 and MDM2 at 12q14.3-q15. Previous CGH studies of OS have revealed abnormalities of chromosome 12, including gains at bands 12p12-p13 , 12q12-q13 , and 12q13-q14 . Expression of the CCND2 gene, which is located at chromosome 12p13, has been observed in various malignancies, including prostate cancer and breast cancer [29–31]. CCND2 encodes a protein belonging to the cyclin family of proteins that regulate cyclin-dependent kinase (CDK) kinases . CDK activity controls the cell cycle G1/S transition by regulating phosphorylation of the tumor suppressor protein Rb . These facts suggest that CCND2 controls proliferation of UTOS-1 tumor cells.
Some studies indicate that 14 to 27% of OS tumors have abnormal MDM2 expression [34, 35]. MDM2 is a target gene of the transcription factor tumor protein p53 . The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop [37, 38]. Overexpression of MDM2 gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. These findings suggest the possible involvement of the p53 tumor suppressor gene, which is associated with development of OS in UTOS-1 cells.
The gain of chromosome band at 17p11.2-p12 has been observed in approximately 13 to 29% of high-grade OS [24, 39, 40]. In UTOS-1 cells, gain of the genes FLI and TOP3A at chromosome 17p11.2-p12 has been observed. These findings suggest that multiple gains, including FLI, TOP3 or other genes close to these candidate oncogenes, are present at chromosome 17p11.2-p12 and contribute to OS tumorigenesis . Recent studies indicate that overexpression of 17p11.2-p12 is associated with p53 degradation [42–44].
In a study of OS using a cDNA array, Squire et al. observed amplification of the genes MYC, GAS7, and PM1 in OS cells . Other reports indicate that losses of chromosome bands 6q16 and 6q21-q22 occur in high-grade OS . These findings and those of the present study suggest that gene losses on chromosome 6q, including HTR1B and D6S268, contribute to OS tumorigenesis.
One of the most remarkable breakpoint clusters that have been found in OS tumors was detected on chromosome 20 by spectral karyotyping (SKY) analysis . Chromosome 20 is one of the smaller chromosomes, suggesting that it is particularly vulnerable to structural rearrangement. However, there is little evidence that chromosome 20 is frequently involved in chromosomal imbalances [26, 28]. In the present study, the only loss that involved chromosome 20 occurred at band 20q13.2-q13.3. Many chromosomal changes have been observed in CGH studies of high-grade OS . Reports indicate that the genes involved in OS tumorigenesis include DAB2 (at chromosome 5q13), OCRL1 (at Xq25), and SHGC17327 (at 18ptel). However, many of these genes were not previously known to be associated with OS tumorigenesis.
In conclusion, we have isolated and characterized a new permanent human cell line, UTOS-1, established from an osteoblastic OS. This cell line retains the morphology, osteoblastic activities and cytogenetic characteristics of the original tumor in vitro. The UTOS-1 cell line is useful for biologic and molecular pathogenetic studies of human OS.
We thank all members of the Department of Orthopaedic Surgery, University of Toyama.
- Meyers PA, Gorlick R, Heller G, Casper E, Lane J, Huvos AG, Healey JH: Intensification of preoperative chemotherapy for osteogenic sarcoma: results of the Memorial Sloan-Kettering (T12) protocol. J Clin Oncol. 1998, 16: 2452-2458.Google Scholar
- Bacci G, Lari S: Current treatment of high grade osteosarcoma of the extremity: review. J Chemother. 2001, 13: 235-243.View ArticleGoogle Scholar
- Uchida A, Myoui A, Araki N, Yoshikawa H, Shinto Y, Ueda T: Neoadjuvant chemotherapy for pediatric osteosarcoma patients. Cancer. 1997, 79: 411-415. 10.1002/(SICI)1097-0142(19970115)79:2<411::AID-CNCR26>3.0.CO;2-2.View ArticleGoogle Scholar
- Fournier B, Price PA: Characterization of a new human osteosarcoma cell line OHS-4. J Cell Biol. 1991, 114: 577-583. 10.1083/jcb.114.3.577.View ArticleGoogle Scholar
- Yamane T: Establishment and characterization of cell lines derived from a human osteosarcoma. Clin Orthop. 1985, 199: 261-271.Google Scholar
- Yoshikawa H, Ohishi M, Kohriki S, Yoshiura M, Ohsaki Y: Establishment and characterization of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1). Oral Oncol. 1997, 33: 163-168. 10.1016/S0964-1955(96)00075-9.View ArticleGoogle Scholar
- Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thompson D, Bar-Shavit Z, Shull S, Mann K, Rodan GA: Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. Cancer Res. 1987, 47: 4961-4966.Google Scholar
- Boehm AK, Squire JA, Bayani J, Nelson M, Neff J, Bridge JA: Cytogenetic findings in 35 osteosarcoma specimens and a review of the literature. Pediatr Pathol Mol Med. 2000, 19: 359-376. 10.1080/15227950050193623.View ArticleGoogle Scholar
- Ozaki T, Neumann T, Wai D, Schäfer KL, van Valen F, Lindner N, Scheel C, Böcker W, Winkelmann W, Dockhorn-Dworniczak B, Horst J, Poremba C: Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping. Cancer Genet Cytogenet. 2003, 140: 145-152. 10.1016/S0165-4608(02)00685-4.View ArticleGoogle Scholar
- Cherrier B, Gouin F, Heymann MF, Thiéry JP, Rédini F, Heymann D, Duteille F: A new experimental rat model of osteosarcoma established by intrafemoral tumor cell inoculation, useful for biology and therapy investigations. Tumor Biol. 2005, 26: 121-130. 10.1159/000086483.View ArticleGoogle Scholar
- Yasuda T, Matsui H, Kanamori M, Yudoh K, Ohmori K, Aoki M, Tsuji H: Effects of tumor cell-derived interleukin 1 alpha on invasiveness of metastatic clones of murine RCT sarcoma through endothelial cells. Tumor Biol. 1999, 20: 105-116. 10.1159/000030053.View ArticleGoogle Scholar
- Carmichael J, DeGraff WG, Gazdar AF: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 47: 936-942.Google Scholar
- Koide O, Iwai S, Kanno T, Kanda S: Isoenzyme of alkaline phosphatase in germinoma cells. Am J Clin Pathol. 1988, 89: 611-616.Google Scholar
- Nishio J, Iwasaki H, Ishiguro M, Ohjimi Y, Yo S, Isayama T, Naito M, Kikuchi M: Supernumerary ring chromosome in a Bednar tumor (pigmented dermatofibrosarcoma protuberans) is composed of interspersed sequences from chromosomes 17 and 22: a fluorescence in situ hybridization and comparative genomic hybridization analysis. Genes Chromosomes Cancer. 2001, 30: 305-309. 10.1002/1098-2264(2000)9999:9999<::AID-GCC1091>3.0.CO;2-R.View ArticleGoogle Scholar
- Shaffer LG, Tommerup N, editors: ISCN. An international system for human cytogenetic Nomenclature. Basel: S Karger. 2005Google Scholar
- Kallioniemi A, Kallioniemi OP, Sudar D, Rutovitz D, Gray JW, Waldman F, Pinkel D: Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992, 258: 818-821. 10.1126/science.1359641.View ArticleGoogle Scholar
- Bilbe G, Roberts E, Birch M, Evans DB: PCR phenotyping of cytokines, growth factors and their receptors and bone matrix proteins in human osteoblast-like cell lines. Bone. 1996, 19: 437-445. 10.1016/S8756-3282(96)00254-2.View ArticleGoogle Scholar
- Rochet N, Dubousset J, Mazeau C, Zanghellini E, Farges MF, de Novion HS, Chompret A, Delpech B, Cattan N, Frenay M, Gioanni J: Establishment, characterization and partial cytokine expression profile of a new human osteosarcoma cell line (CAL 72). Int J Cancer. 1999, 82: 282-285. 10.1002/(SICI)1097-0215(19990719)82:2<282::AID-IJC20>3.0.CO;2-R.View ArticleGoogle Scholar
- Bridge JA, Nelson M, McComb E, McGuire MH, Rosenthal H, Vergara G, Maale GE, Spanier S, Neff JR: Cytogenetic findings in 73 osteosarcoma specimens and a review of the literature. Cancer Genet Cytogenet. 1997, 95: 74-87. 10.1016/S0165-4608(96)00306-8.View ArticleGoogle Scholar
- Murata H, Kusuzaki K, Takeshita H, Hirasawa Y, Ashihara T, Abe T, Inazawa J: Aberrations of chromosomes 1 and 17 in six human osteosarcoma cell lines using double-target fluorescence in situ hybridization. Cancer Genet Cytogenet. 1998, 107: 7-10. 10.1016/S0165-4608(98)00072-7.View ArticleGoogle Scholar
- Wolf M, Tarkkanen M, Hulsebos T, Larramendy ML, Forus A, Myklebost O, Aaltonen LA, Elomaa I, Knuutila S: Characterization of the 17p amplicon in human sarcomas: microsatellite marker analysis. Int J Cancer. 1999, 82: 329-333. 10.1002/(SICI)1097-0215(19990730)82:3<329::AID-IJC4>3.0.CO;2-1.View ArticleGoogle Scholar
- Sandberg AA, Bridge JA: Updates on the cytogenetics and molecular genetics of bone and soft tissue tumors: osteosarcoma and related tumors. Cancer Genet Cytogenet. 2003, 145: 1-30. 10.1016/S0165-4608(03)00105-5.View ArticleGoogle Scholar
- Overholtzer M, Rao PH, Favis R, Lu XY, Elowitz MB, Barany F, Ladanyi M, Gorlick R, Levine AJ: The presence of p53 mutations in human osteosarcomas correlates with high levels of genomic instability. Proc Natl Acad Sci USA. 2003, 100: 11547-11552. 10.1073/pnas.1934852100.View ArticleGoogle Scholar
- Tarkkanen M, Karhu R, Kallioniemi A, Elomaa I, Kivioja AH, Nevalainen J, Böhling T, Karaharju E, Hyytinen E, Knuutila S, Kallioniemi OP: Gains and losses of DNA sequences in osteosarcomas by comparative genomic hybridization. Cancer Res. 1995, 55: 1334-1338.Google Scholar
- Beheshti B, Braude I, Marrano P, Thorner P, Zielenska M, Squire JA: Chromosomal localization of DNA amplifications in neuroblastoma tumors using cDNA microarray comparative genomic hybridization. Neoplasia. 2003, 5: 53-62.View ArticleGoogle Scholar
- Pollack JR, Perou CM, Alizadeh AA, Eisen MB, Pergamenschikov A, Williams CF, Jeffrey SS, Botstein D, Brown PO: Genome-wide analysis of DNA copy-number changes using cDNA microarrays. Nat Genet. 1999, 23: 41-46. 10.1038/14385.View ArticleGoogle Scholar
- Hulsebos TJ, Bijleveld EH, Oskam NT, Westerveld A, Leenstra S, Hogendoorn PC, Bras J: Malignant astrocytoma-derived region of common amplification in chromosomal band 17p12 is frequently amplified in high-grade osteosarcomas. Genes Chromosomes Cancer. 1997, 18: 279-285. 10.1002/(SICI)1098-2264(199704)18:4<279::AID-GCC5>3.0.CO;2-Y.View ArticleGoogle Scholar
- Tarkkanen M, Böhling T, Gamberi G, Ragazzini P, Benassi MS, Kivioja A, Kallio P, Elomaa I, Picci P, Knuutila S: Comparative genomic hybridization of low-grade central osteosarcoma. Mod Pathol. 1998, 11: 421-426.Google Scholar
- Knuutila S, Autio K, Aalto Y: Online access to CGH data of DNA sequence copy number changes. Am J Pathol. 2000, 157: 689-View ArticleGoogle Scholar
- Padar A, Sathyanarayana UG, Suzuki M, Maruyama R, Hsieh JT, Frenkel EP, Minna JD, Gazdar AF: Inactivation of cyclin D2 gene in prostate cancers by aberrant promoter methylation. Clin Cancer Res. 2003, 9: 4730-4734.Google Scholar
- Yu J, Leung WK, Ebert MP, Leong RW, Tse PC, Chan MW, Bai AH, To KF, Malfertheiner P, Sung JJ: Absence of cyclin D2 expression is associated with promoter hypermethylation in gastric cancer. Br J Cancer. 2003, 88: 1560-1565. 10.1038/sj.bjc.6600940.View ArticleGoogle Scholar
- Morgan DO: Principles of Cdk regulation. Nature. 1995, 374: 131-134. 10.1038/374131a0.View ArticleGoogle Scholar
- Weinberg RA: The retinoblastoma protein and cell cycle control. Cell. 1995, 81: 323-330. 10.1016/0092-8674(95)90385-2.View ArticleGoogle Scholar
- Ladanyi M, Cha C, Lewis R, Jhanwar SC, Huvos AG, Healey JH: MDM2 gene amplification in metastatic osteosarcoma. Cancer Res. 1993, 53: 16-18.Google Scholar
- Oliner JD, Kinzler KW, Meltzer PS, George DL, Vogelstein B: Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature. 1992, 358: 80-83. 10.1038/358080a0.View ArticleGoogle Scholar
- Sakamuro D, Sabbatini P, White E, Prendergast GC: The polyproline region of p53 is required to activate apoptosis but not growth arrest. Oncogene. 1997, 15: 887-898. 10.1038/sj.onc.1201263.View ArticleGoogle Scholar
- Kubbutat MH, Jones SN, Vousden KH: Regulation of p53 stability by Mdm2. Nature. 1997, 387: 299-303. 10.1038/387299a0.View ArticleGoogle Scholar
- Candau R, Scolnick DM, Darpino P, Ying CY, Halazonetis TD, Berger SL: Two tandem and independent sub-activation domains in the amino terminus of p53 require the adaptor complex for activity. Oncogene. 1997, 15: 807-816. 10.1038/sj.onc.1201244.View ArticleGoogle Scholar
- Stock C, Kager L, Fink FM, Gadner H, Ambros PF: Chromosomal regions involved in the pathogenesis of osteosarcomas. Genes Chromosomes Cancer. 2000, 28: 329-336. 10.1002/1098-2264(200007)28:3<329::AID-GCC11>3.0.CO;2-F.View ArticleGoogle Scholar
- Zielenska M, Bayani J, Pandita A, Toledo S, Marrano P, Andrade J, Petrilli A, Thorner P, Sorensen P, Squire JA: Comparative genomic hybridization analysis identifies gains of 1p35-36 and chromosome 19 in osteosarcoma. Cancer Genet Cytogenet. 2001, 130: 14-21. 10.1016/S0165-4608(01)00461-7.View ArticleGoogle Scholar
- van Dartel M, Cornelissen PW, Redeker S, Tarkkanen M, Knuutila S, Hogendoorn PC, Westerveld A, Gomes I, Bras J, Hulsebos TJ: Amplification of 17p11.2-p12, including PMP22, TOP3A, and MAPK7 in high-grade osteosarcoma. Cancer Genet Cytogenet. 2002, 139: 91-96. 10.1016/S0165-4608(02)00627-1.View ArticleGoogle Scholar
- van Dartel M, Redeker S, Bras J, Kool M, Hulsebos TJ: Overexpression through amplification of genes in chromosome region 17p11.2-p12 in high-grade osteosarcoma. Cancer Genet Cytogenet. 2004, 152: 8-14. 10.1016/j.cancergencyto.2003.09.024.View ArticleGoogle Scholar
- Henriksen J, Aagesen TH, Maelandsmo GM, Lothe RA, Myklebost O, Forus A: Amplification and overexpression of COPS3 in osteosarcomas potentially target TP53 for proteasome-mediated degradation. Oncogene. 2003, 22: 5358-5361. 10.1038/sj.onc.1206671.View ArticleGoogle Scholar
- van Dartel M, Hulsebos TJ: Amplification and overexpression of genes in 17p11.2-p12 in osteosarcoma. Cancer Genet Cytogenet. 2004, 153: 77-80. 10.1016/j.cancergencyto.2004.03.007.View ArticleGoogle Scholar
- Squire JA, Pei J, Marrano P, Beheshti B, Bayani J, Lim G, Moldovan L, Zielenska M: High-resolution mapping of amplifications and deletions in pediatric osteosarcoma by use of CGH analysis of cDNA microarrays. Genes Chromosomes Cancer. 2003, 38: 215-225. 10.1002/gcc.10273.View ArticleGoogle Scholar
- Tarkkanen M, Elomaa I, Blomqvist C, Kivioja AH, Kellokumpu-Lehtinen P, Böhling T, Valle J, Knuutila S: DNA sequence copy number increase at 8q: a potential new prognostic marker in high-grade osteosarcoma. Int J Cancer. 1999, 84: 114-121. 10.1002/(SICI)1097-0215(19990420)84:2<114::AID-IJC4>3.0.CO;2-Q.View ArticleGoogle Scholar
- Bayani J, Zielenska M, Pandita A, Al-Romaih K, Karaskova J, Harrison K, Bridge JA, Sorensen P, Thorner P, Squire JA: Spectral karyotyping identifies recurrent complex rearrangements of chromosomes 8, 17, and 20 in osteosarcomas. Genes Chromosomes Cancer. 2003, 36: 7-16. 10.1002/gcc.10132.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.