- Open Access
RNAi-mediated knockdown of cyclooxygenase2 inhibits the growth, invasion and migration of SaOS2 human osteosarcoma cells: a case control study
© Zhao et al; licensee BioMed Central Ltd. 2011
- Received: 14 January 2011
- Accepted: 5 March 2011
- Published: 5 March 2011
Cyclooxygenase2 (COX-2), one isoform of cyclooxygenase proinflammatory enzymes, is responsible for tumor development, invasion and metastasis. Due to its role and frequent overexpression in a variety of human malignancies, including osteosarcoma, COX-2 has received considerable attention. However, the function of COX-2 in the pathogenesis of cancer is not well understood. We examined the role of COX-2 in osteosarcoma.
We employed lentivirus mediated-RNA interference technology to knockdown endogenous gene COX-2 expression in human osteosarcoma cells (SaOS2) and analyzed the phenotypical changes. The effect of COX-2 treatment on the proliferation, cell cycle, invasion and migration of the SaOS2 cells were assessed using the MTT, flow cytometry, invasion and migration assays, respectively. COX-2, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) mRNA and protein expression were detected by RT-PCR and western blotting.
Our results indicate that a decrease of COX-2 expression in human osteosarcoma cells significantly inhibited the growth, decreased the invasion and migration ability of SaOS2 cells. In addition, it also reduced VEGF, EGF and bFGF mRNA and protein expression.
The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosarcoma.
- Vascular Endothelial Growth Factor
- Migration Ability
- SaOS2 Cell
- Human Osteosarcoma Cell
Osteosarcoma is the most common primary malignant tumor arising in bone predominantly affecting children and adolescents . It is also one of the most heterogeneous of human tumors . The 5-year survival rate has increased up to 70% in patients with localized disease, however, the prognosis is very poor and the 5-year survival rate is only 20-30% in patients with metastatic disease at diagnosis . Although an adjuvant treatment regimen after surgical resection seems to prolong survival, the precise treatment protocol of drug-of-choice is still debated because the exact mechanisms the development and progression of osteosarcoma are still largely unknown . Effective systemic therapy capable of reversing the aggressive nature of this disease is currently not available . Therefore, an understanding of the molecular mechanisms of osteosarcoma is one of the most important issues for treatment. New therapeutic strategies are necessary to increase survival rates in patients with osteosarcoma.
Cyclooxygenases are key enzymes in the conversion of arachidonic acid into prostaglandin (PG) and other eicosanoids including PGD2, PGE2, PGF2, PGI2 and thromboxane A2 . There are two isoforms of cyclooxygenase, designated COX-1 and COX-2. COX-1 is constitutively expressed in most tissues, and seems to perform physiological functions . However, COX-2 is an inducible enzyme associated with inflammatory disease and cancer. Many reports have indicated that COX-2 expression is increased in a variety of human malignancies, including osteosarcoma, and is responsible for producing large amounts of PGE2 in tumor tissues [8–11]. These molecules are thought to play a critical role in tumor growth, because they reduce apoptotic cell death, stimulate angiogenesis and invasiveness [12, 13]. COX-2 overexpression has been associated with poor prognosis in osteosarcoma . Selective COX-2 inhibitors have been shown to significantly reduce the cell proliferation rates as well as invasiveness in U2OS cells . Transgenic mice overexpressing human COX-2 in mammary glands developed focal mammary gland hyperplasia, dysplasia and metastatic tumors . Epidemiological studies have revealed a decreased risk of colon cancer in people who regularly take COX-2 inhibitors [17, 18]. Specifically, COX-2 silencing mediated by RNA interference (RNAi) has been found to be associated with decreased invasion in laryngeal carcinoma  and human colon carcinoma. In this report, for the first time, we employed RNAi technology to explore the therapeutic potential of the DNA vector-based shRNA targeting COX-2 for the treatment of human osteosarcoma. Moreover, the mechanism underlying inhibition of angiogenesis and metastasis by targeting COX-2 is not fully understood. Another aim of this study was to establish whether there is a direct relationship between COX-2 expression and VEGF, EGF and bFGF production in osteosarcoma cells.
Cell culture and infection
The human osteosarcoma cell line, SaOS2 and 293T cells were purchased from the American Type Culture Collection. Cells were grown in 5% CO2 saturated humidity, at 37°C and cultured in DMEM (Gibco, USA) supplemented with penicillin/streptomycin, 2 mmol/L glutamine and 10% FBS. Cells were subcultured at 9 × 104 cells per well into 6-well tissue culture plates. After 24 h culture, cells were infected with recombinant lentivirus vectors at a multiplicity of infection (MOI) of 40.
Design of shRNA and plasmid preparation
Interfering sequence specified for COX-2 gene
Cell proliferation assay
Cell proliferation was determined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SaOS2 cells were seeded in 96-well culture plates in culture medium at an optimal density (4 × 103 cells per well) in triplicate wells for the parental, LV-Control and LV-COX-2siRNA cells. After 1, 2, 3, 4 and 5 d, cells were stained with 20 ml MTT (5 mg/ml) (Sigma, St Louis, MO, USA) at 37°C for 4 h and subsequently made soluble in 150 ml of DMSO. Absorbance was measured at 490 nm using a microtiter plate reader. Cell growth curves were calculated as mean values of triplicates per group.
Cells were collected and washed with PBS, then centrifuged at 800 r/min and fixed with 70% cold ethanol kept at 4°C overnight. Cells were permeabilized in reagent consisting of 0.5% Triton X-100, 230 μg/ml RNase A and 50 μg/ml propidium iodide in PBS. Samples were kept at 37°C for 30 min, followed by flow cytometry analysis (Becton Dickinson FACScan).
Total RNA was extracted from cultured cells using Trizol reagent (Invitrogen, USA) for reverse transcription. RNA were synthesized to cDNA using Superscript First-Strand Synthesis Kit (Promega, USA) following the manufacturer's protocols. Quantitative real-time polymerase chain reaction (RT-PCR) assays were carried out using SYBR Green Real-Time PCR Master Mix (Toyobo, Osaka, Japan) and RT-PCR amplification equipment using specific primers: COX-2, sense strand 5'-CCCTTGGGTGTCAAAGGTAAA-3', antisense strand 5'-AAACTGATGCGTGAAGTGCTG-3', COX-1, sense strand 5'-ATGCCACGCTCTGGCTACGTG-3', antisense strand 5'-CTGGGAGCCCACCTTGAAGGAGT-3', β-actin, sense strand 5'-GCGAGCACAGAGCCTCGCCTTTG-3', antisense strand 5'-GATGCCGTGCTCGATGGGGTAC-3', VEGFA sense strand 5'-CGTGTACGTTGGTGCCCGCT-3', antisense strand 5'-TCCTTCCTCCTGCCCGGCTC-3', VEGFB sense strand 5'-CCCAGCTGCGTGACTGTGCA-3', antisense strand 5'-TCAGCTGGGGAGGGTGCTCC-3', VEGFC sense strand 5'-TGTTCTCTGCTCGCCGCTGC-3', antisense strand 5'-TGCATAAGCCGTGGCCTCGC-3', EGF sense strand 5'-TGCTCCTGTGGGATGCAGCA-3', antisense strand 5'-GGGGGTGGAGTAGAGTCAAGACAGT-3', bFGF sense strand 5'-CCCCAGAAAACCCGAGCGAGT-3', antisense strand 5'-GGGCACCGCGTCCGCTAATC-3', The expression of interest genes were determined by normalization of the threshold cycle (Ct) of these genes to that of the control β-actin.
Cells were lysed in RIPA buffer (150 mM NaCl, 100 mM Tris-HCl, 1% Tween-20, 1% sodium deoxycholate and 0.1% SDS) with 0.5 mM EDTA, 1 mM PMSF, 10 μg/ml aprotinin and 1 μg/ml pepstatin. Proteins were resolved in SDS-PAGE and transferred to PVDF membranes, which were probed with appropriate antibodies, The immunoreactive protein complexes were detected by enhanced chemiluminescence (Amersham Bioscience, Boston, MA). The specific antibody used: anti-COX-2 antibody (Cell Signaling, #4842, 1 μg/ml), anti-VEGFA antibody (Abcam, ab51745, 0.1 μg/ml), anti-VEGFB antibody (Cell Signaling, #2463, 1 μg/ml), anti-VEGFC antibody (Cell Signaling, #2445, 1 μg/ml), anti-EGF antibody (Cell Signaling, #2963, 1 μg/ml), anti-bFGF antibody (Cell Signaling, #8910, 1 μg/ml), anti-β-actin antibody (Cell Signaling, #4970, 1 μg/ml).
Invasion by SaOS2 cells was assayed using 12-well cell culture chambers containing inserts with 8 μm pores coated with matrigel (Corning, USA). The cells were added to the upper chamber at a density of 4 × 104 cells/insert, After 24 h of incubation, cells on the upper surface were wiped off with a cotton swab. Cells that had invaded the lower surface were fixed with 70% ethanol, stained with 0.2% crystal violet, Invasiveness was quantitated by selecting ten different views (100 times) and calculating the number of invading cells.
Migration assays were performed using two-chamber-Transwell (Corning, USA) as described previously . The lower surface of a polycarbonate filter with 8 μm pores was coated with 1 μg/ml bovine collagen IV. Cells were trypsinized and suspended in a serum-free medium containing 1% BSA at a concentration of 4 × 104 cells/insert. The cells were placed in the upper chamber and free DMEM was placed in the lower chamber. After 12 hr at 37°C, the cells in the upper chamber were wiped off with a cotton swab. The cells on the lower surface of the filter were fixed with 70% ethanol, stained with 0.2% crystal violet, migration was quantitated by selecting ten different views (100 times) and calculating the number of migrated cells.
All statistical analyses were performed using SPSS 10.0. Data were expressed as mean ± SD. The statistical correlation of data between groups was analyzed by one-way analysis of variance (ANOVA) and Student's t test, where P < 0.05 were considered significant.
Selection of the most effective COX-2 specific shRNA expression vector
Downregulation of COX-2 expression by LV-COX-2siRNA-1 in SaOS2 cells
Effects of LV-COX-2siRNA-1 on cell growth of SaOS2 cells
Effects of LV-COX-2siRNA-1 on cell cycle of SaOS2 cells
Cell cycle detected by flow cytometry (%)
48.52 ± 1.38
36.40 ± 1.12
18.0 ± 2.08
46.46 ± 1.56
36.42 ± 1.51
17.12 ± 1.78
58.79 ± 1.54a
25.09 ± 1.16b
16.12 ± 2.16
Effects of LV-COX-2siRNA-1 on invasion and migration ability of SaOS2 cells
Effects of LV-COX-2siRNA-1 on VEGF, EGF and bFGF expression in SaOS2 cells
Many reports have indicated that COX-2 is overexpressed in a variety of human malignancies and is responsible for producing a large quantity of PGE2 in tumor tissues [21–23]. PGE2 stimulates angiogenesis, promotes cell proliferation and invasiveness, and thus it plays a critical role in tumor growth [24, 25]. In addition, COX-2 expression has been found significantly higher in tumors of higher grade and in more aggressive malignancies . Many policies have been employed to inhibit COX-2 expression and function. Dandekar et al pointed out that reduction of COX-2 suppresses tumor growth and improves efficacy of chemotherapeutic drugs in prostate cancer [27–29]. Other groups reported that the COX-2 inhibitors attenuate migration and invasion of breast cancer cells . These data indicate that, as a critical regulator of proliferation of tumor cells, COX-2 is a considerable target for inhibiting growth, triggering apoptosis, and reducing invasion activity.
To this day, there have been many strategies used to inhibit COX-2 expression and activity, including inhibitors and antisense oligonucleotides and RNAi [27, 29, 30]. Selective COX-2 inhibitors both inhibit tumor cell growth and boost chemosensitivity or radiosensitivity of malignancies [31, 32]. To ensure the efficacy and specificity of COX-2 as a therapeutic target, we employed RNAi technology. RNAi refers to the introduction of homologous double stranded RNA (dsRNA) to specifically target a gene's product, resulting in null or hypomorphic phenotypes [33, 34]. It has demonstrated great prospects for studying gene function, signal transduction research and gene therapy. We used RT-PCR and western blotting to proof the efficacy of LV-COX-2siRNA-1 on COX-2 expression in 293T and SaOS2 cells. LV-COX-2siRNA-1 was applied and the expression of COX-2 mRNA and protein were significantly inhibited.
Accumulating evidence has indicated that COX-2 promotes tumor growth, increases cancer cell invasiveness and metastasis through its catalytic activity [35, 36]. Not only COX-2 transfection but also PGE2 treatment enhances cell migration and invasion in various types of human cancers [37–41]. In the present study, the invasion and migration ability of the SaOS2 cells were tested and found that COX-2 gene knockdown by RNAi resulted in a decreased level of invasion and migration. Therefore, there is a strong relationship between COX-2 and the invasion or migration ability of human osteosarcoma cells.
It is well known that the growth of tumor cells depends on nutrition supply, which largely relies on angiogenesis. VEGF plays a key role in normal and abnormal angiogenesis since it stimulates almost every step in the angiogenic process [42, 43]. Other factors that have been shown to stimulate angiogenesis include EGF, bFGF, hepatocyte growth factor, interleukin-8, and placental growth factor [44, 45]. Previous work indicated that COX-2 inhibitors blocked tumor growth via an antiangiogenic mechanism . Moreover, studies demonstrated that there is a strong link between COX-2 expression and tumor angiogenesis . Therefore, COX-2 overexpression may increase tumor blood supply and contribute to tumor growth. Our results suggest that knockdown of the COX-2 gene could suppress invasion and migration ability based on the down-regulation of vegfa, egf and bfgf expression in osteosarcoma cells.
Our experimental data demonstrate that RNAi-mediated downregulation of COX-2 effectively inhibited the cell proliferation, reduced invasion and migration ability of SaOS2 cells with the decreased expression of VEGFA, EGF and bFGF. Although the mechanism of this inhibition needs to be further investigated, our results suggest that COX-2 may have a role in angiogenesis and may be a potential therapeutic target for the treatment of human osteosarcoma.
This research was supported by grants from the Shanghai Health Bureau Science Fund for Young Scholars (2009Y037), the Technology Development Fundation of Shanghai Jiaotong University School of Medicine (09XJ21048).
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