ATF2 predicts poor prognosis and promotes malignant phenotypes in renal cell carcinoma
- Deng-shuang Wu†1,
- Cheng Chen†2,
- Zhen-jie Wu†1,
- Bing Liu1,
- Li Gao3,
- Qing Yang4,
- Wei Chen1, 5,
- Jun-ming Chen1, 6,
- Yi Bao1,
- Le Qu1, 7Email author and
- Lin-hui Wang1Email author
© The Author(s). 2016
Received: 29 February 2016
Accepted: 23 June 2016
Published: 4 July 2016
Activating transcription factor 2 (ATF2) is a basic helix-loop-helix transcription factor, which has been shown to participate in the pathobiology of numerous cancers. However, the role of ATF2 in renal cell carcinoma (RCC) remains unclear.
ATF2 knockdown and overexpression studies were performed in RCC cells to evaluate changes in cell viability, cell cycle, apoptosis, migration and invasion. Xenograft models were used to examine the tumorigenic and metastatic capability of RCC cells upon ATF2 suppression. The expression of ATF2 in human RCC samples was determined using immunohistochemistry on a tissue microarray.
ATF2 knockdown in RCC cells reduced their proliferative and metastatic potentials, whereas ATF2 overexpression enhanced these properties. Mechanistic studies revealed that the transcription of CyclinB1, CyclinD1, Snail and Vimentin was directly regulated by ATF2 in RCC cells. Moreover, ATF2 was shown to be highly expressed in RCC tissues, especially in tumors with metastases. High expression of ATF2 correlated with aggressive clinico-pathological characteristics and predicted poor prognosis of RCC patients.
ATF2 exerts an oncogenic role in RCC and could serve as an important prognostic biomarker.
KeywordsRenal cell carcinoma ATF2 Proliferation Metastasis Prognosis
Renal cell carcinoma (RCC) is the most prevalent malignancy of adult kidney, and its incidence has been increasing in recent decades [1, 2]. Patients with early-stage disease can be effectively treated with surgical resection, however, approximately 20 % of patients present with metastatic disease at the time of initial diagnosis. Moreover, up to 20 % of RCC patients will develop metastases lesions following nephrectomy [3, 4]. Therefore, there is an urgent need to elucidate the molecular mechanisms underlying RCC progression, and identify novel biomarkers and therapeutic targets to improve the prognosis of RCC patients.
Activating transcription factor 2 (ATF2) is a member of ATF family , characterized by a basic structural region and a leucine zipper domain . In response to extracellular stresses or inflammatory cytokines, the transcriptional activity of ATF2 is induced through Thr69- and/or Thr71- phosphorylation by Jun N-terminal kinase (JNK) or p38 (MAPK14) [7–9]. In melanoma and non-small cell lung carcinoma, ATF2 acts as an important oncogene [10, 11], while in nonmalignant skin and breast cancer, ATF2 elicits tumor-suppressor function [12, 13], suggesting a context-dependent role for ATF2 in cancer biology.
Currently, the role of ATF2 in the physiology and pathology of kidney is limited to the evidence that ATF2 has been demonstrated to play a key role in the morphogenesis and apoptosis of renal epithelial cells [14, 15], possibly resulting in renal cyst formation . However, the role of ATF2 in RCC remains unknown. Herein, we examined the effects of ATF2 on the malignant phenotypes of RCC cells, and explored its clinical significance in patients with RCC.
Cell lines and reagents
The human RCC cell lines ACHN and 786-O were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA) and were cultured in MEM (Invitrogen, Carlsbad, CA, USA) and RPMI-1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) respectively, and supplemented with 10 % fetal bovine serum (Gibco, USA). Matrigel was purchased from BD (USA).
Patients and clinical samples
RCC patients who underwent surgery between January 2007 and April 2014 at the Department of Urology, Changzheng Hospital, Second Military Medical University (Shanghai, China) were enrolled in our study (n = 205). The ethical approval was granted from the Committees for Ethical Review at Second Military Medical University and informed consent was obtained from all patients. All specimens were formalin-fixed, paraffin embedded and pathologically confirmed, and subsequently used to construct a tissue microarray. The expression levels of ATF2 were examined by immunohistochemistry.
Paraffin embedded sections were deparaffinized, rehydrated, and then prepared for antigen retrieval. Sections were blocked with 10 % goat serum and incubated with primary antibody against ATF2 (Proteintech-14834-1-AP, dilution 1:50) at 4 °C overnight, and biotin-labeled secondary antibody for 1 h at room temperature. Subsequently, the samples were developed by adding DAB and counterstained with hematoxylin (Beyorime Institute of Biotechnology, Inc.).
Plasmids and cell transfection
Human full-length cDNA of ATF2 was cloned into expression plasmid pCMV3-ATF2-Flag (Sino Biological lnc.). A short hairpin RNA (shRNA) sequence was designed by Hanbio Biotechnology Co. Ltd (Shanghai, China) to target human ATF2 gene (NM_001256090.1). After annealing, double strands of shRNA were inserted into lentiviral pHBLV-U6-Puro vector (Addgene). For lentiviral packaging, HEK-293 T cells were co-transfected with the lentiviral vector, and packaging vectors psPAX2 and PMD2G using LipoFiter™ Liposomal Transfection Reagent (Hanbio, Shanghai, China), according to the manufacturer instructions. RCC cells were seeded in 6-well plates and grown to 50 % confluence on the day of infection. Four hours prior to infection, cells were placed in serum-free media and lentivirus particles were added to the culture medium at a multiplicity of infection (MOI) of 30. Cells were grown at 37 °C, and 24-h following transfection cells were placed with fresh media. The sequences of primers used for plasmid construction in this study were provided in Additional file 1: Table S1.
Transfections were carried out using jetPRIME® - Polyplus-transfection according to the manufacturer’s protocol. One day prior to transfection, cells were seeded in six-well plates. 4 μg of DNA was diluted into 150 mM NaCl and 6 μl of jetPEI® was diluted into 150 mM NaCl to a final volume of 200 μl per well. After 12 h, cells were placed in fresh media.
RNA extraction and quantitative real-time PCR (qRT-PCR)
Total RNA was extracted from cells and tissues using TRIzol reagent (Invitrogen) according to the manufacturer instructions. First-strand cDNA was generated from 2 μg total RNA using the PrimeScript RT reagent kit (Takara, Dalian, China) with random primers. qRT-PCR was performed on an ABI Prism 7300 (Applied Biosystems, Foster City, CA, USA). Sequences of primers used for qRT-PCR in this study were provided in Additional file 1: Table S2. The relative expression level of indicated genes was compared to that of β-Actin and was calculated using the 2−ΔΔCt method. Each qRT-PCR reaction was performed in triplicate.
Western blot analysis
RCC cells were lysed using 1 × SDS sample buffer and heated at 95 °C for 10 min. Quantified proteins were separated on an SDS-PAGE and transferred to PVDF membranes. The membranes were blocked with 5 % nonfat milk for 2 h at room temperature and incubated with specific antibodies. The following primary antibodies were used in our study: ATF2 (Proteintech-14834-1-AP), Phospho-ATF2 (Thr71) (CST-9221), CyclinB1 (CST-12231), CyclinD1 (CST-2978), E-Cadherin (BS-1097), Snail (BS-1853), Vimentin (BS-1491) and β-Actin (Santa cruz-81178). Band intensity was quantitatively analyzed using Quantity One software (Bio-Rad, USA), and the absolute intensity of target protein was normalized to the absolute intensity of β-Actin.
Cell proliferation assay
Cell proliferation was analyzed using the Cell Counting Kit 8 (CCK8, Beyotime Institute of Biotechnology, Shanghai, China). RCC cells were seeded in 96-well plates at a density of 2000 cells per well. At indicated time points, 10 μl CCK8 solution was added into each well and incubated for 2 h. The absorbance at 450 nm was measured to assess the number of viable cells. The results were obtained from three independent experiments in triplicate.
Plate colony formation assay
ACHN cells (500 cells) were seeded into 10 cm plates and cultured in the 37 °C incubator for ~10 days until most single colony were composed of more than 100 cells. The plates were washed by PBS, fixed with 4 % paraformaldehyde, and stained with crystal violet. The number of colonies containing more than 100 cells was counted in each well.
Flow cytometry analysis
Cell apoptosis and cell cycle progression were quantified using flow cytometry analysis (BD Biosciences, San Jose, CA). For apoptosis experiment, RCC cells were collected and washed twice with ice-cold PBS and re-suspended in 200 μl binding buffer. FITC-conjugated Annexin V was added to a final concentration of 0.5 μg/ml and incubated for 20 min at room temperature in the dark, prior to the addition of 1 μg/ml propidium iodide (PI). Samples were immediately analyzed by flow cytometry. For cell cycle progression, RCC cells were collected, washed with PBS and fixed with 75 % ethanol. Cells were stained with PI and RNase overnight at 4 °C. Samples were analyzed by flow cytometry.
RCC cells were seeded at 5 × 105 cells/well in 6-well plates and cultured until the plates were confluent. The cell monolayer was scraped in a straight line using a 10 μl pipette tip to create a scratch, washed with PBS twice and the medium was replaced with serum free medium. Images were captured at 0, 6, and12 h following the initial scratch to evaluate cell migration.
Cell transwell assays were performed with 24-well transwell chamber uncoated (migration) or matrigel-coated (invasion) according to the manufacturer instructions (pore size 8 μm, Corning Life Sciences, NY, USA). 1 × 104 serum pre-starved RCC cells in 250 μl serum free media were seeded into the upper chamber, and the bottom chamber contained medium supplemented with 10 % FBS. After 24 h incubation, the cells on the upper surface of the membrane were scraped off, and the cells on the bottom side of the membrane were fixed with 4 % paraformaldehyde and stained with crystal violet. Cells were counted from 8 randomly chosen fields (magnification, ×200).
Chromatin immunoprecipitation (ChIP)
Following transfection, 786-O cells (1 × 107 cells) were cross-linked with 1 % formaldehyde and incubated for 10 min at 37 °C. ChIP assay was performed according to the manufacturer’s protocol (Millipore, USA) using monoclonal Anti-Flag® M2 antibody produced in mouse (Sigma) or normal rabbit IgG as a negative control (Santa Cruz Biotechnology). An aliquot of lysates (20 μl) was taken out as input control. DNA enrichment was determined by quantitative PCR (qPCR), and was normalized to input. Sequences of primers used for ChIP-qPCR in this study were provided in Additional file 1: Table S3. The products of qPCR were detected by agarose gel electrophoresis.
In vivo tumor xenograft experiment
Male nude mice (BALB/c Nude; 4 weeks old) were purchased from the Shanghai Institute of Material Medical (Chinese Academy of Science) and maintained in a pathogen-free condition in accordance with relevant guidelines and regulations for the care and use of laboratory animals, with the approval of the Institutional Animal Care and Use Committee at Second Military Medical University. ACHN cells (5 × 106 cells) in 100 μl PBS were implanted subcutaneously into the flanks of nude mice. Tumor size was monitored at 3 days intervals using calipers, and the tumor volumes were calculated according to the following formula: tumor volume = largest diameter × perpendicular height2 × 0.5. For lung metastasis model, ACHN cells (1 × 106 cells) in 200 μl PBS were injected into the tail vein of mice. Mice were sacrificed 12 weeks after inoculation and consecutive sections of the whole lung were subjected to hematoxylin-eosin staining. All of the metastatic lesions in lung were calculated microscopically to evaluate the development of pulmonary metastasis.
Apoptosis in tumor tissues was detected by DNA fragmentation with an apoptosis detection kit (Roche TUNEL-Apo-AP; USA) according to the manufacturer instruction. Tissues were deparaffinized and hydrated as previously described. The samples were developed by adding DAB and counterstained with hematoxylin (Beyorime Institute of Biotechnology, Inc.). Images were taken with a Nikon microscope at 200× magnifications.
Statistical analysis was performed with SPSS Statistics software version 19 (SPSS Inc., USA). Data were presented as “mean ± SD”. Pearson chi-square test was used to analyze the clinical variables. Kaplan-Meier method and log-rank tests were used to compare RCC patient survival based on dichotomized ATF2 expression. Cox proportional hazards regression analysis were used to analyze the independent factors on the survival prognosis of patients with RCC. Differences between groups were analyzed by Student’s t-test after evaluating the normal distribution of results. A p-value < 0.05 was considered statistically significant.
ATF2 is highly expressed in RCC tissues
ATF2 promotes RCC cells proliferation by promoting Cyclin expression
We then evaluated the cell cycle distribution by flow cytometry. Knockdown of ATF2 increased the proportion of cells in G0/G1 phase, with concomitant decrease in S and G2/M phase (Fig. 2c). These results indicated that ATF2 may promote cell cycle progression. In addition, suppression of ATF2 enhanced the ratio of early and late apoptotic cells (Fig. 2d).
Conversely, exogenous expression of ATF2 in RCC cells (Additional file 1: Figure S1C and 1D) enhanced cell proliferation and induced an increase in the number of cells in S phase compared to control cells (Fig. 2e-2g).
Cell cycle is precisely regulated by cyclins, we therefore evaluated the effect of ATF2 on their expressions. Notably, the mRNA and protein expression of CyclinB1 and CyclinD1 were downregulated upon ATF2 knockdown (Additional file 1: Figure S2A and Fig. 2h) and upregulated upon ATF2 overexpression (Additional file 1: Figure S2B and Fig. 2i). Moreover, ChIP assay demonstrated that ATF2 was enriched on the proximal promoters of CyclinB1 and CyclinD1 in cells overexpressing ATF2 (Fig. 2j), indicating that ATF2 bound to the promoter of CyclinB1 and CyclinD1 and promoted their transcription. Taken together, these results revealed that ATF2 promotes RCC cells proliferation at least in part by transactivating the expression of CyclinB1 and CyclinD1.
ATF2 facilitates RCC cell migration and invasion
Epithelial to mesenchymal transition (EMT) plays a pivotal role in tumor metastasis [18, 19]. We thus examined the effect of ATF2 on the expression of EMT markers. Increased levels of epithelial markers (E-Cadherin) and decreased levels of mesenchymal markers (Snail and Vimentin) were detected in RCC cells following ATF2 knockdown (Additional file 1: Figure S2C and Fig. 3f). Conversely, ATF2 overexpression decreased E-Cadherin expression and increased the expression of Snail and Vimentin (Additional file 1: Figure S2D and Fig. 3g). Furthermore, we observed an enrichment of ATF2 at the proximal promoter region of Snail and Vimentin upon ATF2 overexpression (Fig. 3h). Thus, these results suggest that ATF2 promotes RCC metastasis partially depending on EMT induction.
ATF2 knockdown suppresses tumor growth and metastasis in vivo
To examine the effect of ATF2 on RCC metastasis in vivo, we established a lung metastasis model. As shown in Fig. 4g, RCC cells with ATF2 knockdown formed decreased number and size of pulmonary metastatic lesions in mice.
High levels of ATF2 predict poor prognosis of RCC patients
Emerging evidence has indicated that ATF2 exerts paradoxical roles in different cancers . For instance, ATF2 acts as a tumor suppressor in breast cancer by promoting the expression of the apoptosis-regulated gene GADD45α and suppressor gene Maspin . Similarly, the deletion of ATF2 in keratinocytes upregulated of β-catenin and downregulated of Notch consequently facilitated skin tumor formation . By contrast, inhibition of ATF2 repressed tumorigenesis of melanoma via increasing JNK/Jun and JunD activities . Therefore, the role of ATF2 may depend on cancer-specific contexts and its role in RCC remains unknown. In this study, we reported the oncogenic role of ATF2 in the regulation of RCC growth and metastasis both in vitro and in vivo. By using a large cohort of RCC patients, we identified ATF2 as a novel biomarker to predict RCC prognosis.
Cell proliferation needs cell cycle progression, which is known to be controlled by cyclins. Cyclin B1 and Cyclin D1 are key regulators for G1/S and G2/M transition respectively [23–26], depletion of which inhibited proliferation and induced apoptosis in many human tumors . ATF2 has been reported to modify Cyclin B1 and Cyclin D1 levels to promote cell-cycle progression in several types of neoplasms, including cervical and breast cancer [27, 28]. Our results further revealed the enrichment of ATF2 at the proximal promoter region of Cyclin B1 and Cyclin D1, suggesting that ATF2 promotes the transcription of Cyclin B1 and Cyclin D1 to enhance RCC cell proliferation.
EMT plays a pivotal role in tumor metastasis and contributes to early-stage dissemination of cancer cells [29–31]. ATF2 was reported to promote metastasis by regulating EMT in solid tumors such as pancreatic cancer . However, this relationship has never been demonstrated in RCC. Herein, we found that depletion of ATF2 caused a conversion from mesenchymal to epithelial phenotype, marked by upregulation of E-cadherin and downregulation of Snail and Vimentin. Furthermore, we found that Snail and Vimentin were direct downstream targets of ATF2 in RCC cells, suggesting that ATF2 enhanced metastasis of RCC cells by the induction of EMT.
As a transcription factor, the divergent roles of ATF2 may be associated with differential subcellular localization . In the present study, we found that ATF2 expression was higher in RCC samples and primarily localized in the nucleus of RCC cells (Fig. 1c), suggesting the transcriptional function of ATF2. Consistent with the oncogenic role of ATF2 in functional study, RCC patients with high ATF2 expression were associated with aggressive clinico-pathological characteristics. Moreover, high ATF2 level was significantly correlated with decreased survival in RCC patients, and could serve as an independent predictor for poor prognosis.
In summary, ATF2 plays a critical role in promoting cell proliferation and metastasis of RCC, and could serve as an independent predictor for the clinical outcome in RCC patients. Based on these findings, targeting ATF2 may represent a potential therapeutic strategy to curb the progression of RCC.
ATF2, activating transcription factor 2; CCK8, cell counting kit 8; ChIP, chromatin immunoprecipitation; DFS, disease free survival; EMT, epithelial to mesenchymal transition; IHC, immunohistochemistry; JNK, Jun N-terminal kinase; MOI, multiplicity of infection; OS, overall survival; PI, propidium iodide; qPCR, quantitative PCR; qRT-PCR, quantitative real-time PCR; RCC, renal cell carcinoma; shRNA, short hairpin RNA
This work was supported by grants from the National Natural Science Foundation of China (81272817, 81572521), Youth Talent YangFan Program of Shanghai Science and Technology Committee (16YF1403600) and the Leading Talent Project of Shanghai (2013046). The authors disclose no potential conflicts of interest.
DSW and LQ conducted all experiments and analyzed the data. WC, JMC and YB provided clinical samples. LG and QY provided pathology evaluation. CC provided support with experimental techniques. ZJW collected clinical data. DSW and LQ wrote the manuscript. BL contributed to manuscript revision. LHW and LQ conceived the project and supervised all experiments. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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