Correction: Hippo component YAP promotes focal adhesion and tumour aggressiveness via transcriptionally activating THBS1/FAK signaling in breast cancer

Correction: J Exp Clin Cancer Res 37, 175 (2018)

https://doi.org/10.1186/s13046-018-0850-z

Following the publication of the original article [1], errors were found in Figs. 2i and 6i. Incorrect adhesion assay photo of “siYAP-2#” group into “siYAP-3#” group in Fig. 2i and the Transwell photo of “siTHBS1-1#” group into the “siTHBS1-2#” group in Fig. 6i were presented.

The authors declare that the correction does not change the results or conclusions of this paper. The correct figures are given below:

Fig. 2

YAP was able to induced cell migration, invasion and focal adhesion in breast cancer cell lines. (a) Western blot verified the overexpression of YAP in MCF7 cells. EV: empty vector; o/e: overexpression. (b) Western blot verified the knockdown of YAP in MDA-MB-231 cells via a collection of siRNAs; siYAP-#2 and siYAP-#3 has relatively high knockdown efficiency, thus these two siRNAs were used in this research. (c, d) Transwell assay showing that overexpression of YAP induced cell migration and invasion ability in MCF7 cells. The experiment was performed in triplicate. **p < 0.01 by Student’s t-test. Scale bar: 100 μm. (e, f) Transwell assay showing that knockdown of YAP significantly inhibited cell migration and invasion ability in MDA-MB-231 cells. The experiment was performed in triplicate. ** p < 0.01 by ANOVA test. Scale bar: 100 μm. (g, h) Overexpression of YAP induced MCF7 cell adhesion to gelatin. The attached cells were stained with Wright’s-Giemsa and are shown in (g). The experiment was performed in triplicate. ** p < 0.01 by Student’s t-test. Scale bar: 100 μm. (i, j) Knockdown of YAP significantly inhibited MDA-MB-231 cell adhesion to gelatin. The attached cells were stained with Wright’s-Giemsa and are shown in (i). The experiment was performed in triplicate. ** p < 0.01 by Student’s t-test. Scale bar: 100 μm. (k) Overexpression of YAP induced focal adhesions in MCF7 cells. Focal adhesions were visualized by co-localization of paxilin (green) and F-actin (stained with phalloidin, red). Nuclei were counterstained with DAPI (blue). Scale bar: 20 μm. (l) Knockdown of YAP expression inhibited focal adhesions in MDA-MB-231 cells. Scale bar: 20 μm. (m) Quantification of the membrane-localized paxilin in (k). The experiment was performed in triplicate. ** p < 0.01 by Student’s t-test. (n) Quantification of the membrane-localized paxilin in (l). The experiment was performed in triplicate. ** p < 0.01 by ANOVA test

Fig. 6

YAP triggered FAK phosphorylation and focal adhesion through THBS1. (a) Western blot assays revealed that knockdown of THBS1 expression in MCF7-YAP-S127A cells could significantly reverse FAK Y397 phosphorylation. (b) Cell adhesion assays showed that knockdown of THBS1 could significantly reverse YAP-S127A-induced cell adhesion in MCF7 cells. The experiments were performed in triplicate. Scale bar: 100 μm. (c) Transwell invasion assays showed that knockdown of THBS1 could significantly reverse YAP-S127A-induced cell invasion in MCF7 cells. The experiments were performed in triplicate. Scale bar: 100 μm. (d) Quantification of the cell adhesion ability in (b). * p < 0.05 and ** p < 0.01 by ANOVA test. (e) Quantification of the cell invasion ability in (c). ** p < 0.01 by ANOVA test. (f) Knockdown of THBS1 inhibited focal adhesion in MCF7-YAP-S127A cells. Red: F-actin (stained with phalloidin); Green: paxilin; Blue: nucleus (stained with DAPI). Scale bar: 20 μm. (g) Knockdown of THBS1 reduced FAK Y397 phosphorylation in MDA-MB-231 cells. (h) Knockdown of THBS1 expression reduced cell adhesion to gelatin in MDAMB-231 cells. The experiments were performed in triplicate. Scale bar: 100 μm. (i) Transwell invasion assays showed that knockdown of THBS1 expression reduced cell invasion in MDA-MB-231 cells. The experiments were performed in triplicate. Scale bar: 100 μm. (j) Quantification of the cell adhesion ability in (h). ** p < 0.01 by ANOVA test. (k) Quantification of the cell invasion ability in (i). ** p < 0.01 by ANOVA test. (l) Knockdown of THBS1 reduced focal adhesion in MDA-MB-231 cells. Red: F-actin (stained with phalloidin); Green: paxilin; Blue: nucleus (stained with DAPI). Scale bar: 20 μm. (m) Model for how YAP regulates THBS1 expression and induces focal adhesion