- Open Access
IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer
- Puja Kachroo†1, 2, 3,
- Mi-Heon Lee†1, 3,
- Ling Zhang1, 3,
- Felicita Baratelli1, 2,
- Gina Lee1, 2,
- Minu K Srivastava1, 4,
- Gerald Wang1, 2,
- Tonya C Walser1, 2,
- Kostyantyn Krysan1, 2,
- Sherven Sharma1, 4,
- Steven M Dubinett1, 2, 4 and
- Jay M Lee1, 2, 3, 5Email author
© Kachroo et al.; licensee BioMed Central Ltd. 2013
- Received: 14 August 2013
- Accepted: 22 November 2013
- Published: 25 November 2013
Interleukin-27 signaling is mediated by the JAK-STAT pathway via activation of STAT1 and STAT3, which have tumor suppressive and oncogenic activities, respectively. Epithelial–mesenchymal transition (EMT) and angiogenesis are key processes in carcinogenesis. Although IL-27 has been shown to have potent anti-tumor activity in various cancer models, the role of IL-27 in EMT and angiogenesis is poorly understood. In this study, we investigated the role of IL-27 in regulating EMT and angiogenesis through modulation of the STAT pathways in human non-small cell lung carcinoma (NSCLC) cells.
STAT activation following IL-27 exposure was measured in human NSCLC cell lines. Expression of epithelial (E-cadherin, γ-catenin) and mesenchymal (N-cadherin, vimentin) markers were assessed by Western blot analysis. Production of pro-angiogenic factors (VEGF, IL-8/CXCL8, CXCL5) were examined by ELISA. Cell motility was examined by an in vitro scratch and transwell migration assays. Selective inhibitors of STAT1 (STAT1 siRNAs) and STAT3 (Stattic) were used to determine whether both STAT1 and STAT3 are required for IL-27 mediated inhibition of EMT and secretion of angiogenic factors.
Our results demonstrate that IL-27 stimulation in NSCLC resulted in 1) STAT1 and STAT3 activation in a JAK-dependent manner, 2) development of epithelial phenotypes, including a decrease in the expression of a transcriptional repressor for E-cadherin (SNAIL), and mesenchymal marker (vimentin) with a reciprocal increase in the expression of epithelial markers, 3) inhibition of cell migration, and 4) reduced production of pro-angiogenic factors. STAT1 inhibition in IL-27–treated cells reversed the IL-27 effect with resultant increased expression of Snail, vimentin and the pro-angiogenic factors. The inhibition of STAT3 activation had no effect on the development of the epithelial phenotype.
IL-27 induces mesenchymal to epithelial transition and inhibits the production of pro-angiogenic factors in a STAT1–dominant pathway. These findings highlight the importance of STAT1 in repressing lung carcinogenesis and describe a new anti-tumor mechanism of IL-27.
Interleukin-27 (IL-27) is a member of the IL-12 cytokine family known to have both pro-inflammatory and anti-inflammatory functions . In preclinical models, IL-27 has been shown to have anti-tumor properties in a variety of malignancies through several mechanisms, including inhibition of tumor proliferation and angiogenesis [2–8]. IL-27 has attracted interest as an anti-tumor agent because of its similarities to IL-12, which also demonstrated ability to suppress tumor growth and elicit tumor specific immune responses . However, the use of IL-12 as a single agent has been limited by its toxicity and poor response in clinical trials for advanced renal or ovarian cancers necessitating studies in other promising agents [9, 10].
IL-27 elicits its effects through activation of both STAT1 and STAT3, which have opposing roles in carcinogenesis [1, 2, 8, 11–15]. Activated STAT1 signaling has tumor suppressive roles by inhibiting angiogenesis, tumor growth and metastasis as well as promoting apoptosis [12, 16]. Alternatively, the STAT3 pathway has been shown to be constitutively activated in many human cancers and has been implicated in oncogenic transformation and progression [17–21]. IL-27 is a heterodimeric molecule, composed of Epstein-Barr virus-induced gene 3 (EBI3) and p28 subunits, that is expressed by activated antigen presenting cells . The intracellular component of its receptor, comprised of glycoprotein 130 (gp130) and WSX-1 (also known as IL-27Rα or TCCR), associates with cytoplasmic protein kinases such as JAKs (Janus Activated Kinases) that mediate cytokine signaling . The JAK-Signal Transducer and Activator of Transcription (STAT) signaling pathway, which was initially identified as a critical process in normal cellular processes, has also been implicated in tumor initiation and malignant progression. The STAT transcriptional factors, which are phosphorylated by the JAKs, dissociate from the receptor and dimerize followed by nuclear translocation .
Epithelial-mesenchymal transition (EMT) is an evolutionarily conserved process in which cells undergo conversion from an epithelial to mesenchymal phenotype whereby cells develop loose cell-cell interactions and become motile . The importance of EMT in driving carcinogenesis has been shown in lung, breast, prostate, pancreatic, and liver cancers [25, 26].
IL-27 mediated inhibition of angiogenesis is a known anti-tumor mechanism in various malignancies [3, 5]. Although a study showed that either over-expression or treatment with recombinant IL-27 led to anti-tumor activity on murine and human lung cancer cells, there is limited insight on the mechanism that modulates EMT and angiogenesis . Furthermore, the mechanisms by which IL-27 plays a role in modulation of EMT and angiogenesis in NSCLC through the STAT pathways have not been studied. On this basis and given the fact that IL-27 regulates STAT transcriptional factors (STAT1 and STAT3) that possess opposing activities in cancer, the impact of this cytokine on lung carcinogenesis was investigated. Here, we report that IL-27 promotes the expression of epithelial markers, inhibits cell migration and the production of angiogenic factors in human NSCLC through a STAT1 dominant pathway. To our knowledge, the antitumor activity of IL-27 through a STAT1 dependent pathway has not been previously described.
Materials and methods
Cell lines and culture
Human NSCLC cell lines (A549, H2122, H1703, H292, H1437, H460, H1650, and H358) were obtained from the American Type Culture Collection (Rockville, MD). The H157 cell line was obtained from the National Cancer Institute (Bethesda, MD). Cells were verified by genotyping and tested for Mycoplasma. The cancer cells lines were maintained in RPMI-1640 with L-glutamine (Hyclone, Logan, UT) supplemented with 5% fetal bovine serum (FBS; Gemini Bio-products, West Sacramento, CA) in a humidified atmosphere of 5% CO2 at 37°C.
Recombinant human IL-27 (R&D Systems, Inc, Minneapolis, MN) was added at a concentration of 50 ng/mL in serum-free medium. JAK inhibitor I (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) binds to the JAK2 kinase domain and inhibits JAK1, JAK2, and JAK3. It was reconstituted in DMSO and added at various concentrations from 1-100 nM in serum-free medium. STAT3 inhibitor V, Stattic (Santa Cruz Biotechnology, Inc, Santa Cruz, CA), is a nonpeptidic small molecule that selectively inhibits the SH2 domain of STAT3, thereby blocking its phosphorylation and dimerization. It was dissolved in DMSO and used at a concentration of 7.5 nM in serum-free medium. Opti-MEM I Reduced Serum-Medium and Lipofectamine 2000 reagents (Invitrogen, Carlsbad, CA) were utilized for transfection.
A549 cells were stained with anti-human IL-27 Rα/WSX-1/TCCR-PE or isotype control (R&D systems, Minneapolis, MN) for 30 min at room temperature and analyzed by FACSCalibur (BD, San Jose, CA). FACS data were analyzed using Flowjo software (Treestar, Ashland, OR).
Transfection of STAT1 small interfering RNA into A549 cells
Cells were seeded in 6-well plates and grown to 40-50% confluence at the time of transfection. For each sample, 2.5 μL of siRNA (10 μM) was diluted in 200 μL of Opti-MEM I. Two different constructs of STAT1 siRNA (Cell Signaling Technology, Danvers, MA) were used to inhibit STAT1 and a non-targeting siRNA (Ambion, Carlsbad, CA) was used as a control siRNA. The sequences for the STAT1 siRNAI and STAT1 siRNAII are 5’-CGAGAGCUGUCUAGGUUAAC-3' and 5'- GGGCAUCAUGCAUCUUACU-3', respectively. Similarly, 2.5 μL of Lipofectamine 2000 was diluted in 200 μL of Opti-MEM I. After 5 minutes of incubation at room temperature, the diluted oligomers were combined with the diluted Lipofectamine 2000 and incubated for 30 minutes at room temperature. The oligomer-Lipofectamine 2000 complexes were then added to each well containing the cells and medium and mixed gently. The cells were then incubated at 37°C in a CO2 incubator for 6 hours after which the wells were washed and further cultured for 18 hours after replaced with serum-free medium. The cells were then treated with IL-27 and/or Stattic per experimental design.
Cell lysates were prepared with RadioImmunoPrecipitation Assay (RIPA) buffer (PBS, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS) containing protease inhibitors on ice after washing with PBS and were centrifuged at 13,000 rpm for 20 minutes at 4°C. Protein concentrations of cell lysates were measured by BCA assay and up to 20 μg of total protein were used for each SDS-PAGE. Western blot was performed after transferring SDS-PAGE gels to Amersham Hybond-ECL membranes (GE Healthcare, Piscataway, NJ). After incubation with 5% nonfat milk or BSA in TBST (10 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Tween 20) for 1 hour at room temperature, the membrane was incubated with antibodies against phosphorylated-STAT1 (Tyr 701,1:1000), total-STAT1(1:1000), phosphorylated-STAT3 (Tyr 705, 1:1000 dilution), total-STAT3 (1:1000 dilution), Snail (1:1000) (Cell Signaling Technology, Danvers, MA), and Vimentin (1:2000) (BD Biosciences, San Jose, CA) at 4°C for overnight, and N-cadherin (1:5000), γ-catenin (1:7000), E-cadherin (1:6000), (BD Biosciences, San Jose, CA), and GAPDH (1:10,000) (Advanced ImmunoChemical, Long Beach, CA) at room temperature at 1 hour. Membranes were washed three times for 10 min and incubated with a 1:10,000 dilution of horseradish peroxidase-conjugated anti-mouse or anti-rabbit antibodies (Santa Cruz Biotechnology, Dallas, Texas). Blots were washed with TBST three times and developed with the ECL system (PerkinElmer, Waltham, MA) according to the manufacturer’s protocols.
Enzyme-linked immunosorbent assay (ELISA)
ELISA kits for human vascular endothelial growth factor (VEGF), IL-8/CXLC8, and CXCL5 were used (R&D Systems, Minneapolis, MN). Concentrations of human VEGF, IL-8/CXCL8 and CXCL5 in culture supernatant were measured by ELISA following kit instructions. Briefly, 100 μL of the samples were loaded on the plates and incubated for 2 hours at room temperature. After the plates were washed with wash buffer (0.05% Tween20 in PBS), they were incubated with detection antibody for 2 hours at room temperature. Immunoreactivity was determined by adding substrate solution and absorbance (450 nm) was determined by Vmax Kinetic microplate reader (Molecular Devices, Sunnyvale, CA).
In vitro cell motility assay
Cancer cells were plated in 6-well flat-bottom plates and allowed to adhere overnight. After serum starvation, cells were subject to different treatment conditions. Once the cells reached 90-95% confluence, a 200 μL pipette tip was used to make a scratch in the monolayer of cells in each well. The same fields were observed for cell migration using a phase-contrast microscope and photographed at various time points for up to 60 hours.
Transwell cell migration assay
A549 cells were cultured to 40-60% confluence on glass coverslips (ThermoFisher Scientific, Waltham, MA), allowed to adhere overnight, and placed in serum free medium for four hours prior to IL-27 exposure for 15 minutes at 37°C. The cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 minutes at room temperature and then permeabilized with methanol for 15 minutes at -20°C. After blocking with 5% BSA in PBS solution for 1 hour at room temperature, the coverslips were incubated with primary antibody (1:100 dilution) overnight at 4°C. The following day, the coverslips were incubated with fluorescein-conjugated goat anti-rabbit IgG secondary antibody (1:50 dilution; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 30 minutes at room temperature followed by the addition of a DAPI (4'-6-Diamidino-2-phenylindole) nuclear stain (1:2000 dilution) for 2 minutes at room temperature. ProLong Gold antifade reagent (Invitrogen) was placed on the coverslip and the cells were then observed under the microscope. The ImageJ (Image Processing and Analysis in Java) program was used to create the merged immunofluorescence images.
Statistical significance was determined using the two-tailed Student’s t test and p values less than 0.05 were considered significant.
IL-27 activates STAT1 and STAT3 with resultant translocation to the nucleus in human NSCLC cells
To validate this concept in other histological subtypes of NSCLC, seven additional human lung cancer cell lines (H1703, H292, H157, H1437, H460, H1650, and H358) were exposed to IL-27 for 24 hours and P-STAT1 and P-STAT3 protein levels were analyzed by Western blot. Similar to A549 cells, all cell lines, with the exception of H460 and H358, demonstrated activation of both transcriptional factors P-STAT1 and P-STAT3 following IL-27 stimulation (Figure 1B). Total STAT1 and STAT3 levels were comparable in H157, H1437, H460 and H358 cells. There were increased levels of total STAT1 and STAT3 in H1703 and H292, while decreased in H358 cells, The basis for differential expression of the total STATs in response IL-27 stimulation in lung cancer cells is unclear, but may be related to known underlying mutational heterogeneity of different cancer cell lines .
The tyrosine phosphorylated forms of STAT transcriptional factors are known to translocate to the nucleus for regulation of gene transcription . Immunofluorescence microscopy further confirmed STAT1 (Figure 1C) and STAT3 (Figure 1D) protein activation and nuclear translocation in A549 cells. In the absence of IL-27, there were no detectable levels of phosphorylated STAT1 or STAT3 in A549 cells (upper left, Figure 1C and 1D). In contrast, IL-27-treated A549 cells showed phosphorylation of STAT1 and STAT3 following 15 minutes of exposure to IL-27 (upper right, Figure 1C and 1D), with translocation into the nucleus as demonstrated by the overlay of FITC and DAPI staining (bottom right, Figure 1C and 1D).
Next, we tested whether IL-27 treatment affects expression levels of the IL-27 receptor on A549 cells. FACS analysis of A549 cells showed that these cells express substantial amounts of IL-27 receptor (TCCR) on the cell surface (Figure 1E). However, the presence of IL-27 did not affect expression levels of IL-27 receptor on A549 cells at 24 hours (Figure 1F). Evaluation for IL-27 receptor expression at earlier time points (15 minutes, 30 minutes, 1 hour, and 2 hours) was not changed by IL-27 stimulation (data not shown). These results demonstrate that IL-27 activates STAT1 and STAT3 with resultant translocation into the nucleus without altering expression levels of the IL-27 receptor.
IL-27-mediated STAT activation requires JAK activation
IL-27 regulates and prevents over-expression of STAT3 through activation of the STAT1 pathway
The specificity of STAT activation is determined by the presence of the docking sites on the receptor, and STAT1 and STAT3 have been shown to be activated in response to gp130 receptor activation by various stimuli [29, 30]. STAT1 and STAT3 are known to regulate transcription of target genes playing opposing roles in tumorigenesis . In order to determine if a dominant STAT pathway becomes activated by IL-27, we performed selective inhibition of the STAT1 or STAT3 pathways.
Given the interdependence of STAT1 and STAT3 activation following IL-27 stimulation, STAT3 inhibition was evaluated by adding Stattic, a nonpeptidic small molecule that inhibits the function of the SH2 domain required for tyrosine phosphorylation, dimerization and subsequent nuclear translocation of STAT3 . The STAT3 inhibitor was added to A549 cells for 1 hour prior to IL-27 exposure for 15 or 30 minutes and the expression of activated and total amounts of STAT1 and STAT3 proteins were analyzed by Western blot. As expected, the expression of P-STAT3 was markedly reduced by pretreatment of STAT3 inhibitor at both time points of IL-27 treatment without affecting T-STAT3 (Figure 3B). However, activated or total amount of STAT1 protein was not significantly changed in the pre-treated cells with Stattic when compared with untreated cells, indicating that inhibition of STAT3 alone does not have a considerable impact on STAT1 activation. These results suggest that although IL-27 activates both STAT1 and STAT3, the regulation and prevention of over-expressing phosphorylated STAT3 requires the presence of activated STAT1 in NSCLC cells.
IL-27 induces an epithelial phenotype in lung cancer cells through STAT1 activation
A fundamental event during EMT is the loss of cell polarity, resulting in transition of polarized epithelial cells into mobile mesenchymal cells . To evaluate the phenotypic changes of NSCLC cells in response to differential STAT1 and STAT3 activation following IL-27 treatment, changes in morphologic features of lung cancer cells were assessed. In comparison to untreated cells (upper left, Figure 3C), IL-27-treated cells exhibited a more epithelial phenotype characterized by a markedly more cohesive and organized appearance of the cells in a cobblestone monolayer formation (lower left, Figure 3C). Suppression of STAT1 expression by siRNA prior to IL-27 treatment resulted in a phenotype characterized by elongated spindle-shaped, fibroblast-like cells that were morphologically similar to untreated cells (lower middle, Figure 3C), while STAT1 siRNA single treatment did not significantly affect the phenotype of untreated cells (upper middle, Figure 3C). The addition of the STAT3 inhibitor (Stattic) did not demonstrate marked morphologic changes in A549 cells when compared to IL-27- treated or -untreated cells (lower right and upper right, Figure 3C). These findings suggest that STAT1 activation is the dominant pathway by which IL-27 mediates polarization of NSCLC cells towards an epithelial phenotype.
IL-27 promotes expression of epithelial markers through a STAT1 dominant pathway
Next, we examined whether IL-27 induces MET through STAT pathways by blocking STAT1 and STAT3 pathways using STAT1 siRNA or STAT3 inhibitor, Stattic, respectively. As shown in Figure 4, pretreatment with STAT1 siRNA dramatically inhibited expression of T-STAT1, resulting in complete inhibition of STAT1 phosphorylation. Pretreatment with STAT1 siRNA before IL-27 exposure resulted in increased Snail expression, decreased expression of epithelial markers (E-cadherin and γ-catenin), and up regulation of mesenchymal marker (vimentin) compared to treatment with IL-27 alone. STAT1 siRNA mediated down regulation of E-cadherin expression was partially inhibited by the combined treatment with Stattic and STAT1 siRNA given the increased E-cadherin expression when comparing IL-27 + STAT1 siRNA vs. IL-27 + STAT1 siRNA + Stattic groups (Figure 4). These findings suggest that Stattic may directly attenuate the STAT1 siRNA effect on E-cadherin expression. As expected, the total amount of STAT3 protein (T-STAT3) was not changed by Stattic, an inhibitor of STAT3 phosphorylation, but STAT3 phosphorylation was remarkably decreased (Figure 4). When compared to treatment with IL-27 alone, pretreatment with Stattic before IL-27 stimulation did not affect expression of epithelial markers (E-cadherin and γ-catenin) and mesenchymal marker (vimentin), suggesting that STAT1 pathway plays a critical role in the IL-27 mediated regulation of EMT.
Interestingly, there was no significant change in the expression of mesenchymal marker, N-cadherin, by STAT1 inhibition (STAT1 siRNA pretreatment) and the reduced expression of N-cadherin by IL-27 was reversed by STAT3 inhibition (pretreatment with STAT3 inhibitor) (Figure 4), indicating that the decreased expression of N-cadherin by IL-27 may be mediated by STAT3 activation. The decreased expression of Snail by IL-27 was not reversed by inhibition of STAT3 activation.
The mechanism driving the differential effect of IL-27 on the two mesenchymal markers (N-cadherin and Vimentin) is unclear as selective inhibition of STAT1 or STAT3 did not elucidate a clear mechanism (Figure 4). Instead, there was suggestion that STAT3 may be involved in N-cadherin expression (Figure 4). Although N-cadherin is considered a mesenchymal marker, its function may be more complex as other studies have shown that repression of N-cadherin is required for epithelial to mesenchymal transition in some instances such as neural crest migration [34, 38]. However, the overall effect with IL-27 stimulation in our study was promotion of mesenchymal to epithelial transition. The impact of N-cadherin and STAT3 in this process is unclear.
Overall, these results suggest that the STAT3 pathway is not critically involved in the IL-27 mediated promotion of epithelial marker expression. In summary, STAT1 appears to be the dominant pathway by which IL-27 promotes the expression of epithelial markers.
Of note, the reciprocal increase in P-STAT3 compared to control with inhibition of STAT1 by siRNA seen in Figure 3A is not demonstrated in Figure 4. These are two different experiments where the duration of IL-27 stimulation and time point for measurement of P-STAT3 expression are entirely different for the two figures.
IL-27 inhibition of in vitro cell migration is mediated by a STAT3-independent and STAT1-dependent pathway
Cell migration was further studied using the transwell chamber migration assay in which the results were consistent with scratch/wound assay findings. The addition of IL-27 inhibited transwell cell migration (Figure 5D). Treatment with STAT1 siRNA with or without IL-27 significantly increased transwell cell migration compared to control siRNA group (Figure 5D). As such, STAT1 siRNA prevented IL-27 mediated inhibition of cell migration. In contrast, the addition of Stattic showed a significant inhibition of cell migration (Figure 5E). Taken together, our results demonstrate that IL-27 inhibits in vitro cell migration via a STAT1 dependent mechanism and that STAT3 does not appear to be essential in the inhibitory effect.
IL-27-mediated inhibition of angiogenic factors is STAT1-dependent
Tumor growth and metastasis are integrally dependent on production of angiogenic factors and angiogenesis [39–41]. Vascular endothelial growth factor (VEGF) is well known potent angiogenic factor . In addition to VEGF, IL-8/CXCL8 and CXCL5 have been identified as important pro-angiogenic proteins in human NSCLC [43, 44]. It has previously been shown that IL-27 has anti-angiogenic activity by down regulating the expression of VEGF, IL-8/CXCL8 and CXCL5 in human multiple myeloma cells . In this study, we examined the production of pro-angiogenic factors, VEGF, IL-8/CXCL8, and CXCL5, to determine the effects of IL-27 on angiogenesis in human lung cancer.
STAT1 and STAT3 are known to have opposing roles in VEGF regulation. For example, STAT1 has been shown to be a negative regulator of VEGF and angiogenesis [16, 45, 46]. In contrast, STAT3 transactivation with other factors is required for full induction of the VEGF promoter in cancer cells . Similarly, STAT1 is required for inhibition of IL-8 expression mediated by other cytokines . Constitutive activation or knockdown of STAT3 has been shown to up regulate or suppress IL-8 production in human melanoma cells, respectively . The role of STAT1 and STAT3 pathways in the production of CXCL5 in cancer has not been well studied.
The impact of the STAT3 pathway was also studied by the addition of Stattic to the IL-27-treated cells. Pretreatment with the inhibitor of STAT3 activation did not reverse the inhibitory effect of IL-27 on the production of VEGF, IL-8/CXCL8, and CXCL5, but rather led to further decrease in the production of IL-8/CXCL8 when compared to IL-27 alone (Figure 6B, 6D, and 6F), suggesting that IL-27 mediated inhibitory effect on the production of pro-angiogenic factors associated with angiogenesis is independent of STAT3.
Inhibition of both STAT1 and STAT3 activation also reversed the reduction of IL-8 and CXCL5 by IL-27 treatment as demonstrated by the significantly increased expression compared to IL-27 alone (Figure 6D and 6F). The combined STAT1 and STAT3 inhibition effect of reciprocal increased IL-8 and CXCL5 levels did not impact VEGF (Figure 6B). These results suggest that STAT1-dependent inhibitory effect of IL-27 on the production of VEGF may also require STAT3 activation.
Overall, our findings support that STAT1, but not STAT3, plays a primary role in inhibition of pro-angiogenic factor production in human lung cancer by IL-27 treatment. Furthermore, inhibition of STAT1 results in augmentation of pro-angiogenic factors beyond the basal level possibly due to increased STAT3 activation in addition to STAT1 inhibition as shown in Figure 3A. Our data suggests that the impact of basal STAT1 expression may regulate STAT3 activation to control angiogenesis.
Epithelial to mesenchymal transition and angiogenesis have emerged as integral processes in promoting carcinogenesis . The change from epithelial to mesenchymal phenotype has been associated with tumor invasion, metastasis, and unfavorable prognosis . The role of STAT pathways in regulating EMT during carcinogenesis and embryogenesis has been described in a limited number of studies. For example, STAT1 and STAT5 have been shown to be involved in regulating EMT during renal tubule formation and in mammary gland growth and epithelial differentiation, respectively [52, 53]. In cancer, STAT3 has been implicated in EGF-mediated EMT in ovarian cancer cell lines and STAT1 has been reported to inhibit angiogenesis in murine fibrosarcoma tumor cells .
Epithelial and mesenchymal marker expression is known to be important in EMT. Factors such as E-cadherin, catenins, vimentin, and snail have all been correlated with clinical and pathological features in non-small-cell lung cancer [54–56]. Transcriptional repression of E-cadherin by Snail is closely correlated with EMT and the loss of E-cadherin expression is a hallmark of EMT . The expression of E-cadherin and catenins is reduced in NSCLC [55, 56]. In addition, vimentin is over-expressed in many epithelial cancers, including lung cancer, and its over expression in cancer correlates with tumor growth, invasion, and poor prognosis .
IL-27 has been shown to have non-immune antitumor effects in lung cancer that include suppression of COX-2 and PGE2, reduction of vimentin levels, and inhibition of cell migration and invasion . This study showed that IL-27 treatment in lung cancer cells led to increased E-cadherin expression and decreased expression of vimentin and Snail with inhibition of cell migration by suppression of cyclooxygenase-2-mediated activities .
The importance of IL-27 in modulating EMT through the STAT pathways is poorly understood in carcinogenesis. To our knowledge, there have been no studies that have described MET as an anti-tumor mechanism of IL-27. In our study, we hypothesized that IL-27 inhibits EMT and angiogenesis through STAT dependent pathways.
Our results revealed that IL-27-treated lung cancer cells show increased epithelial marker (E-cadherin and γ-catenin), decreased Snail (transcriptional repressor of E-cadherin), and decreased mesenchymal marker (N-cadherin and vimentin) expression. In addition, IL-27 treatment suppressed in vitro cell migration. The ability of IL-27 to promote MET and inhibit cell migration was abolished by inhibition of the STAT1 pathway, but not the STAT3 pathway, with the exception of N-cadherin expression. The impact of N-cadherin and STAT3 in this process is unclear.
Overall, our findings suggest that IL-27 promotes MET and the increased expression of epithelial marker proteins is STAT1-dependent. The inhibition of EMT through STAT1 dependence is a novel anti-tumor mechanism of IL-27, which has not been previously described.
Our results support the body of evidence that STAT1 is associated with tumor suppressive properties, such as inhibition of angiogenesis, tumor growth and metastasis as well as promotion of apoptosis [12, 16]. The role of STAT3 in IL-27 regulation of EMT is not well understood. In present study, the inhibition of STAT3 activation did not reverse the increased expression of epithelial markers (E-cadherin and γ-catenin) and the reduced expression of mesenchymal marker (vimentin) and Snail by IL-27, and STAT3 activation was not required for the inhibition of cell migration by IL-27. Interestingly, the inhibition of STAT1 activation led to increased STAT3 activation in IL-27 treated lung cancer cells whereas inhibition of STAT3 activation alone did not significantly impact STAT1 expression. The current study does not provide a mechanism by which inhibition of STAT1 led to increased STAT3 activation. However, similar to our results, previous studies have demonstrated that STAT1- deficient cells showed increased STAT3 activation [59–61]. Potential mechanisms by which STAT1 may directly inhibit STAT3 include competition for receptor docking sites, promoters of target DNA sequences, and/or binding cofactors. The receptor docking site is a prerequisite for activation by tyrosine phosphorylation and STAT3 can be phosphorylated by receptor bound tyrosine kinases [62, 63]. In fact, it has been shown that STAT1 suppresses STAT3 tyrosine phosphorylation that mediates downstream signaling of other cytokine receptors . Thus it appears likely that STAT1 suppresses IL27-mediated STAT3 activation at least in part by competing for the STAT docking site within the IL-27 receptor cytoplasmic domain.
Our results also demonstrated that the inhibition of STAT1 pathway in IL-27 treated cells resulted in augmented cell migration and increased production of pro-angiogenic factors (VEGF, IL-8, and CXCL-5) compared to untreated cells. These findings may be due to the enhanced STAT3 activation in the setting of inhibition of STAT1 activation. Activated STAT3 has been shown to play an important role in oncogenic transformation and progression in many human cancers [13–15, 17–20]. STAT3 has been shown to regulate cell migration, motility and invasion [64–66] and induce VEGF expression . The anti-angiogenesis properties of IL-27 in tumor models have been described previously. It has been shown that anti-tumor and anti-angiogenic activities of IL-27 in murine melanoma tumors . Cocco et al. described anti-angiogenic properties of IL-27 in a multiple myeloma tumor model . However, these studies did not define the mechanism of IL-27 mediated inhibition of angiogenesis. The augmented cell migration and promotion of angiogenesis factors may be due to the reciprocal increase of STAT3 activation in the setting of STAT1 inhibition. This hypothesis of STAT1 and STAT3 interdependence is further supported by other reports using a genomic technique to map transcriptional factor binding sites and identified STAT3 as a direct transcriptional target of STAT1 . It has also been shown that STAT3 was activated in a sustained strong manner in STAT1 knock-out murine fibroblasts [60, 68]. On this basis, basal STAT1 activation may be required in repressing STAT3 activation.
Cytokines, such as IL-27, that possess divergent functions may play a pivotal role in influencing immune regulation and carcinogenesis through differential STAT1 and STAT3 activation and cross-regulation. There have been limited reports understanding the regulation of EMT in carcinogenesis through STAT pathways. Although the anti-tumor properties of IL-27 have been described previously, our study describes a new mechanism by which IL-27 inhibits EMT and angiogenesis through a STAT1 dominant pathway.
We report that IL-27-mediated induction of MET and inhibition of angiogenic factors is STAT1-dependent, and inhibition of STAT1 activity results in induction of a mesenchymal phenotype and angiogenic factors above basal levels implicating an overwhelming STAT3 effect. These findings suggest that STAT1 activation may play an important role in repressing STAT3 in lung carcinogenesis, and suggest that better understanding of STAT signaling by cytokines such as IL-27 may shed light to potential new targets in cancer prevention and therapy.
This work was supported in part by funding from the Pulmonary and Critical Care Medicine Training Program (NIH/NHLBI 5T32HL072752), NCI (L30 CA142223-01 and 1 K23 CA131577-01A1), Thoracic Surgery Foundation for Research and Education (TSFRE) Grant, Jonsson Cancer Center Foundation, STOP Cancer Research Award, the Ronald Binder Memorial Fund for Lung Cancer Research, NIH/NCATS (UL1TR000124), and Medical Research Funds from the Department of Veterans Affairs (Merit Review 5I01BX000359 and 1I01CX000345-01).
- Villarino AV, Huang E, Hunter CA: Understanding the pro- and anti-inflammatory properties of IL-27. J Immunol. 2004, 173 (2): 715-720.View ArticleGoogle Scholar
- Salcedo R, Stauffer JK, Lincoln E, Back TC, Hixon JA, Hahn C, Shafer-Weaver K, Malyguine A, Kastelein R, Wigginton JM: IL-27 mediates complete regression of orthotopic primary and metastatic murine neuroblastoma tumors: role for CD8+ T cells. J Immunol. 2004, 173 (12): 7170-7182.View ArticleGoogle Scholar
- Cocco C, Giuliani N, Di Carlo E, Ognio E, Storti P, Abeltino M, Sorrentino C, Ponzoni M, Ribatti D, Airoldi I: Interleukin-27 acts as multifunctional antitumor agent in multiple myeloma. Clin Cancer Res. 2010, 16 (16): 4188-4197. 10.1158/1078-0432.CCR-10-0173.View ArticleGoogle Scholar
- Chiyo M, Shimozato O, Yu L, Kawamura K, Iizasa T, Fujisawa T, Tagawa M: Expression of IL-27 in murine carcinoma cells produces antitumor effects and induces protective immunity in inoculated host animals. Int J Cancer. 2005, 115 (3): 437-442. 10.1002/ijc.20848.View ArticleGoogle Scholar
- Shimizu M, Shimamura M, Owaki T, Asakawa M, Fujita K, Kudo M, Iwakura Y, Takeda Y, Luster AD, Mizuguchi J, et al: Antiangiogenic and antitumor activities of IL-27. J Immunol. 2006, 176 (12): 7317-7324.View ArticleGoogle Scholar
- Hisada M, Kamiya S, Fujita K, Belladonna ML, Aoki T, Koyanagi Y, Mizuguchi J, Yoshimoto T: Potent antitumor activity of interleukin-27. Cancer Res. 2004, 64 (3): 1152-1156. 10.1158/0008-5472.CAN-03-2084.View ArticleGoogle Scholar
- Oniki S, Nagai H, Horikawa T, Furukawa J, Belladonna ML, Yoshimoto T, Hara I, Nishigori C: Interleukin-23 and interleukin-27 exert quite different antitumor and vaccine effects on poorly immunogenic melanoma. Cancer Res. 2006, 66 (12): 6395-6404. 10.1158/0008-5472.CAN-05-4087.View ArticleGoogle Scholar
- Yoshimoto T, Morishima N, Mizoguchi I, Shimizu M, Nagai H, Oniki S, Oka M, Nishigori C, Mizuguchi J: Antiproliferative activity of IL-27 on melanoma. J Immunol. 2008, 180 (10): 6527-6535.View ArticleGoogle Scholar
- Hurteau JA, Blessing JA, DeCesare SL, Creasman WT: Evaluation of recombinant human interleukin-12 in patients with recurrent or refractory ovarian cancer: a gynecologic oncology group study. Gynecol Oncol. 2001, 82 (1): 7-10.View ArticleGoogle Scholar
- Motzer RJ, Rakhit A, Thompson JA, Nemunaitis J, Murphy BA, Ellerhorst J, Schwartz LH, Berg WJ, Bukowski RM: Randomized multicenter phase II trial of subcutaneous recombinant human interleukin-12 versus interferon-alpha 2a for patients with advanced renal cell carcinoma. J Interferon Cytokine Res. 2001, 21 (4): 257-263. 10.1089/107999001750169934.View ArticleGoogle Scholar
- Darnell JE: STATs and gene regulation. Science. 1997, 277 (5332): 1630-1635. 10.1126/science.277.5332.1630.View ArticleGoogle Scholar
- Stephanou A, Latchman DS: STAT-1: a novel regulator of apoptosis. Int J Exp Pathol. 2003, 84 (6): 239-244.View ArticleGoogle Scholar
- Huang M, Page C, Reynolds RK, Lin J: Constitutive activation of stat 3 oncogene product in human ovarian carcinoma cells. Gynecol Oncol. 2000, 79 (1): 67-73. 10.1006/gyno.2000.5931.View ArticleGoogle Scholar
- Bromberg JF, Wrzeszczynska MH, Devgan G, Zhao Y, Pestell RG, Albanese C, Darnell JE: Stat3 as an oncogene. Cell. 1999, 98 (3): 295-303. 10.1016/S0092-8674(00)81959-5.View ArticleGoogle Scholar
- Levy DE, Inghirami G: STAT3: a multifaceted oncogene. Proc Natl Acad Sci USA. 2006, 103 (27): 10151-10152. 10.1073/pnas.0604042103.View ArticleGoogle Scholar
- Huang S, Bucana CD, Van Arsdall M, Fidler IJ: Stat1 negatively regulates angiogenesis, tumorigenicity and metastasis of tumor cells. Oncogene. 2002, 21 (16): 2504-2512. 10.1038/sj.onc.1205341.View ArticleGoogle Scholar
- Deng JY, Sun D, Liu XY, Pan Y, Liang H: STAT-3 correlates with lymph node metastasis and cell survival in gastric cancer. World J Gastroenterol. 2010, 16 (42): 5380-5387. 10.3748/wjg.v16.i42.5380.View ArticleGoogle Scholar
- Niu G, Wright KL, Huang M, Song L, Haura E, Turkson J, Zhang S, Wang T, Sinibaldi D, Coppola D, et al: Constitutive Stat3 activity up-regulates VEGF expression and tumor angiogenesis. Oncogene. 2002, 21 (13): 2000-2008. 10.1038/sj.onc.1205260.View ArticleGoogle Scholar
- Horiguchi A, Oya M, Shimada T, Uchida A, Marumo K, Murai M: Activation of signal transducer and activator of transcription 3 in renal cell carcinoma: a study of incidence and its association with pathological features and clinical outcome. J Urol. 2002, 168 (2): 762-765. 10.1016/S0022-5347(05)64741-6.View ArticleGoogle Scholar
- Chang KC, Wu MH, Jones D, Chen FF, Tseng YL: Activation of STAT3 in thymic epithelial tumours correlates with tumour type and clinical behaviour. J Pathol. 2006, 210 (2): 224-233. 10.1002/path.2041.View ArticleGoogle Scholar
- David D, Rajappan LM, Balachandran K, Thulaseedharan JV, Nair AS, Pillai RM: Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis. J Exp Clin Cancer Res. 2011, 30: 56-10.1186/1756-9966-30-56.View ArticleGoogle Scholar
- Hunter CA: New IL-12-family members: IL-23 and IL-27, cytokines with divergent functions. Nat Rev Immunol. 2005, 5 (7): 521-531. 10.1038/nri1648.View ArticleGoogle Scholar
- Leonard WJ, O'Shea JJ: Jaks and STATs: biological implications. Annu Rev Immunol. 1998, 16: 293-322. 10.1146/annurev.immunol.16.1.293.View ArticleGoogle Scholar
- Hay ED: The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it. Dev Dyn. 2005, 233 (3): 706-720. 10.1002/dvdy.20345.View ArticleGoogle Scholar
- Hugo H, Ackland ML, Blick T, Lawrence MG, Clements JA, Williams ED, Thompson EW: Epithelial–mesenchymal and mesenchymal–epithelial transitions in carcinoma progression. J Cell Physiol. 2007, 213 (2): 374-383. 10.1002/jcp.21223.View ArticleGoogle Scholar
- Lee TK, Poon RT, Yuen AP, Ling MT, Kwok WK, Wang XH, Wong YC, Guan XY, Man K, Chau KL, et al: Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition. Clin Cancer Res. 2006, 12 (18): 5369-5376. 10.1158/1078-0432.CCR-05-2722.View ArticleGoogle Scholar
- Ho MY, Leu SJ, Sun GH, Tao MH, Tang SJ, Sun KH: IL-27 directly restrains lung tumorigenicity by suppressing cyclooxygenase-2-mediated activities. J Immunol. 2009, 183 (10): 6217-6226. 10.4049/jimmunol.0901272.View ArticleGoogle Scholar
- Lawrence MS, Stojanov P, Polak P, Kryukov GV, Cibulskis K, Sivachenko A, Carter SL, Stewart C, Mermel CH, Roberts SA, et al: Mutational heterogeneity in cancer and the search for new cancer-associated genes. Nature. 2013, 499 (7457): 214-218. 10.1038/nature12213.View ArticleGoogle Scholar
- Reddy EP, Korapati A, Chaturvedi P, Rane S: IL-3 signaling and the role of Src kinases, JAKs and STATs: a covert liaison unveiled. Oncogene. 2000, 19 (21): 2532-2547. 10.1038/sj.onc.1203594.View ArticleGoogle Scholar
- Ernst M, Jenkins BJ: Acquiring signalling specificity from the cytokine receptor gp130. Trends Genet. 2004, 20 (1): 23-32. 10.1016/j.tig.2003.11.003.View ArticleGoogle Scholar
- Fiszer-Kierzkowska A, Vydra N, Wysocka-Wycisk A, Kronekova Z, Jarzab M, Lisowska KM, Krawczyk Z: Liposome-based DNA carriers may induce cellular stress response and change gene expression pattern in transfected cells. BMC Mol Biol. 2011, 12: 27-10.1186/1471-2199-12-27.View ArticleGoogle Scholar
- Song L, Turkson J, Karras JG, Jove R, Haura EB: Activation of Stat3 by receptor tyrosine kinases and cytokines regulates survival in human non-small cell carcinoma cells. Oncogene. 2003, 22 (27): 4150-4165. 10.1038/sj.onc.1206479.View ArticleGoogle Scholar
- Schust J, Sperl B, Hollis A, Mayer TU, Berg T: Stattic: a small-molecule inhibitor of STAT3 activation and dimerization. Chem Biol. 2006, 13 (11): 1235-1242. 10.1016/j.chembiol.2006.09.018.View ArticleGoogle Scholar
- Kalluri R, Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 2009, 119 (6): 1420-1428. 10.1172/JCI39104.View ArticleGoogle Scholar
- Strutz F, Zeisberg M, Ziyadeh FN, Yang CQ, Kalluri R, Muller GA, Neilson EG: Role of basic fibroblast growth factor-2 in epithelial-mesenchymal transformation. Kidney Int. 2002, 61 (5): 1714-1728. 10.1046/j.1523-1755.2002.00333.x.View ArticleGoogle Scholar
- Cano A, Perez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, Portillo F, Nieto MA: The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol. 2000, 2 (2): 76-83. 10.1038/35000025.View ArticleGoogle Scholar
- Vernon AE, LaBonne C: Tumor metastasis: a new twist on epithelial-mesenchymal transitions. Curr Biol. 2004, 14 (17): R719-R721. 10.1016/j.cub.2004.08.048.View ArticleGoogle Scholar
- Thiery JP: Epithelial-mesenchymal transitions in development and pathologies. Curr Opin Cell Biol. 2003, 15 (6): 740-746. 10.1016/j.ceb.2003.10.006.View ArticleGoogle Scholar
- Carmeliet P, Jain RK: Angiogenesis in cancer and other diseases. Nature. 2000, 407 (6801): 249-257. 10.1038/35025220.View ArticleGoogle Scholar
- Folkman J: Role of angiogenesis in tumor growth and metastasis. Semin Oncol. 2002, 29 (6 Suppl 16): 15-18.View ArticleGoogle Scholar
- Tonini T, Rossi F, Claudio PP: Molecular basis of angiogenesis and cancer. Oncogene. 2003, 22 (42): 6549-6556. 10.1038/sj.onc.1206816.View ArticleGoogle Scholar
- Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J: Vascular-specific growth factors and blood vessel formation. Nature. 2000, 407 (6801): 242-248. 10.1038/35025215.View ArticleGoogle Scholar
- Arenberg DA, Kunkel SL, Polverini PJ, Glass M, Burdick MD, Strieter RM: Inhibition of interleukin-8 reduces tumorigenesis of human non-small cell lung cancer in SCID mice. J Clin Invest. 1996, 97 (12): 2792-2802. 10.1172/JCI118734.View ArticleGoogle Scholar
- Arenberg DA, Keane MP, DiGiovine B, Kunkel SL, Morris SB, Xue YY, Burdick MD, Glass MC, Iannettoni MD, Strieter RM: Epithelial-neutrophil activating peptide (ENA-78) is an important angiogenic factor in non-small cell lung cancer. J Clin Invest. 1998, 102 (3): 465-472. 10.1172/JCI3145.View ArticleGoogle Scholar
- Battle TE, Lynch RA, Frank DA: Signal transducer and activator of transcription 1 activation in endothelial cells is a negative regulator of angiogenesis. Cancer Res. 2006, 66 (7): 3649-3657. 10.1158/0008-5472.CAN-05-3612.View ArticleGoogle Scholar
- von Marschall Z, Scholz A, Cramer T, Schafer G, Schirner M, Oberg K, Wiedenmann B, Hocker M, Rosewicz S: Effects of interferon alpha on vascular endothelial growth factor gene transcription and tumor angiogenesis. J Natl Cancer Inst. 2003, 95 (6): 437-448. 10.1093/jnci/95.6.437.View ArticleGoogle Scholar
- Gray MJ, Zhang J, Ellis LM, Semenza GL, Evans DB, Watowich SS, Gallick GE: HIF-1alpha, STAT3, CBP/p300 and Ref-1/APE are components of a transcriptional complex that regulates Src-dependent hypoxia-induced expression of VEGF in pancreatic and prostate carcinomas. Oncogene. 2005, 24 (19): 3110-3120. 10.1038/sj.onc.1208513.View ArticleGoogle Scholar
- Laver T, Nozell SE, Benveniste EN: IFN-beta-mediated inhibition of IL-8 expression requires the ISGF3 components Stat1, Stat2, and IRF-9. J Interferon Cytokine Res. 2008, 28 (1): 13-23. 10.1089/jir.2007.0062.View ArticleGoogle Scholar
- Oka M, Sakaguchi M, Okada T, Nagai H, Ozaki M, Yoshioka T, Inoue H, Mukaida N, Kikkawa U, Nishigori C: Signal transducer and activator of transcription 3 upregulates interleukin-8 expression at the level of transcription in human melanoma cells. Exp Dermatol. 2010, 19 (8): e50-e55.View ArticleGoogle Scholar
- Sanchez-Garcia I: The crossroads of oncogenesis and metastasis. N Engl J Med. 2009, 360 (3): 297-299. 10.1056/NEJMcibr0808031.View ArticleGoogle Scholar
- Dohadwala M, Yang SC, Luo J, Sharma S, Batra RK, Huang M, Lin Y, Goodglick L, Krysan K, Fishbein MC, et al: Cyclooxygenase-2-dependent regulation of E-cadherin: prostaglandin E(2) induces transcriptional repressors ZEB1 and snail in non-small cell lung cancer. Cancer Res. 2006, 66 (10): 5338-5345. 10.1158/0008-5472.CAN-05-3635.View ArticleGoogle Scholar
- Liu X, Robinson GW, Wagner KU, Garrett L, Wynshaw-Boris A, Hennighausen L: Stat5a is mandatory for adult mammary gland development and lactogenesis. Genes Dev. 1997, 11 (2): 179-186. 10.1101/gad.11.2.179.View ArticleGoogle Scholar
- Kim M, O'Brien LE, Kwon SH, Mostov KE: STAT1 is required for redifferentiation during Madin-Darby canine kidney tubulogenesis. Mol Biol Cell. 2010, 21 (22): 3926-3933. 10.1091/mbc.E10-02-0112.View ArticleGoogle Scholar
- Sato M, Shames DS, Hasegawa Y: Emerging evidence of epithelial-to-mesenchymal transition in lung carcinogenesis. Respirology. 2012, 17 (7): 1048-1059. 10.1111/j.1440-1843.2012.02173.x.View ArticleGoogle Scholar
- Kase S, Sugio K, Yamazaki K, Okamoto T, Yano T, Sugimachi K: Expression of E-cadherin and beta-catenin in human non-small cell lung cancer and the clinical significance. Clin Cancer Res. 2000, 6 (12): 4789-4796.Google Scholar
- Pirinen RT, Hirvikoski P, Johansson RT, Hollmen S, Kosma VM: Reduced expression of alpha-catenin, beta-catenin, and gamma-catenin is associated with high cell proliferative activity and poor differentiation in non-small cell lung cancer. J Clin Pathol. 2001, 54 (5): 391-395. 10.1136/jcp.54.5.391.View ArticleGoogle Scholar
- Iwatsuki M, Mimori K, Yokobori T, Ishi H, Beppu T, Nakamori S, Baba H, Mori M: Epithelial-mesenchymal transition in cancer development and its clinical significance. Cancer Sci. 2010, 101 (2): 293-299. 10.1111/j.1349-7006.2009.01419.x.View ArticleGoogle Scholar
- Satelli A, Li S: Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol Life Sci. 2011, 68 (18): 3033-3046. 10.1007/s00018-011-0735-1.View ArticleGoogle Scholar
- Gil MP, Bohn E, O'Guin AK, Ramana CV, Levine B, Stark GR, Virgin HW, Schreiber RD: Biologic consequences of Stat1-independent IFN signaling. Proc Natl Acad Sci USA. 2001, 98 (12): 6680-6685. 10.1073/pnas.111163898.View ArticleGoogle Scholar
- Qing Y, Stark GR: Alternative activation of STAT1 and STAT3 in response to interferon-gamma. J Biol Chem. 2004, 279 (40): 41679-41685. 10.1074/jbc.M406413200.View ArticleGoogle Scholar
- Ramana CV, Gil MP, Han Y, Ransohoff RM, Schreiber RD, Stark GR: Stat1-independent regulation of gene expression in response to IFN-gamma. Proc Natl Acad Sci USA. 2001, 98 (12): 6674-6679. 10.1073/pnas.111164198.View ArticleGoogle Scholar
- Kerr IM, Costa-Pereira AP, Lillemeier BF, Strobl B: Of JAKs, STATs, blind watchmakers, jeeps and trains. FEBS Lett. 2003, 546 (1): 1-5. 10.1016/S0014-5793(03)00411-3.View ArticleGoogle Scholar
- Ihle JN, Kerr IM: Jaks and Stats in signaling by the cytokine receptor superfamily. Trends Genet. 1995, 11 (2): 69-74. 10.1016/S0168-9525(00)89000-9.View ArticleGoogle Scholar
- Sano S, Itami S, Takeda K, Tarutani M, Yamaguchi Y, Miura H, Yoshikawa K, Akira S, Takeda J: Keratinocyte-specific ablation of Stat3 exhibits impaired skin remodeling, but does not affect skin morphogenesis. EMBO J. 1999, 18 (17): 4657-4668. 10.1093/emboj/18.17.4657.View ArticleGoogle Scholar
- Lim CP, Phan TT, Lim IJ, Cao X: Stat3 contributes to keloid pathogenesis via promoting collagen production, cell proliferation and migration. Oncogene. 2006, 25 (39): 5416-5425. 10.1038/sj.onc.1209531.View ArticleGoogle Scholar
- Ng DC, Lin BH, Lim CP, Huang G, Zhang T, Poli V, Cao X: Stat3 regulates microtubules by antagonizing the depolymerization activity of stathmin. J Cell Biol. 2006, 172 (2): 245-257. 10.1083/jcb.200503021.View ArticleGoogle Scholar
- Bhinge AA, Kim J, Euskirchen GM, Snyder M, Iyer VR: Mapping the chromosomal targets of STAT1 by Sequence Tag Analysis of Genomic Enrichment (STAGE). Genome Res. 2007, 17 (6): 910-916. 10.1101/gr.5574907.View ArticleGoogle Scholar
- Ramana CV, Kumar A, Enelow R: Stat1-independent induction of SOCS-3 by interferon-gamma is mediated by sustained activation of Stat3 in mouse embryonic fibroblasts. Biochem Biophys Res Commun. 2005, 327 (3): 727-733. 10.1016/j.bbrc.2004.12.074.View ArticleGoogle Scholar
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