Pim-1 acts as an oncogene in human salivary gland adenoid cystic carcinoma
- Xin Zhu†1,
- Jia-jie Xu†2,
- Si-si Hu2,
- Jian-guo Feng1,
- Lie-hao Jiang2,
- Xiu-xiu Hou2,
- Jun Cao2,
- Jing Han1,
- Zhi-qiang Ling1 and
- Ming-hua Ge2Email author
© Zhu et al.; licensee BioMed Central. 2014
Received: 11 August 2014
Accepted: 15 December 2014
Published: 31 December 2014
Pim-1 (Provirus integration site for Moloney murine leukemia virus 1) belongs to the Ser/Thr kinase family and plays a pivotal role in occurrence and development of oncogenesis. Recent studies have demonstrated that Pim-1 phosphorylates RUNX3 and alters its subcellular localization. However, few studies have concerned the implications of Pim-1 in the salivary gland adenoid cystic carcinoma (ACC). In this study, we aimed to clarify the function of Pim-1 in ACC in vitro. Meanwhile, we measured the levels of Pim-1 and RUNX3 in the ACC tissues. The correlations between Pim-1/RUNX3 levels and clinical parameters were also analyzed.
SACC-83 and SACC-LM cells were transfected with the Pim-1 siRNA. Pim-1 mRNA and protein expression were measured using real-time PCR and immnuoblot, respectively. Cell proliferation was analyzed by CCK-8 assay. Cell cycle, apoptosis, and mitochondrial membrane potential were detected by flow cytometry. Effects of Pim-1 on cells’ invasion were evaluated by transwell migration assay. Pim-1 and RUNX3 levels in ACC tissues were examined by immunohistochemistry.
Pim-1 siRNA reduces cell proliferation, induces apoptosis, causes cell cycle arrest through cell cycle related proteins (Cyclin D1 and CDK4), mitochondrial depolarization, and decreases invasive ability in SACC-83 and SACC-LM cells. Pim-1 and RUNX3 levels are significantly relevant and associated with T-stage and nerve invasion in the ACC tissues.
This study demonstrates the oncogenic role of Pim-1 in ACC. The findings also suggest that Pim-1 may serve as a neoteric therapeutic target and potential prognostic marker for ACC cancer.
Salivary gland adenoid cystic carcinoma (ACC) accounts for approximately 10% of all epithelial salivary tumors. Clinical characteristics of ACC include high aggressive to nerve and vessel, high rate of recurrence, and frequent metastasis to lung. The 5-year survival rate of patients with highly metastatic ACC is less than 20%. Since ACC is not sensitive to radiotherapy or chemotherapy, surgical resection is the most common treatment for ACC. Like most other tumors, the interaction of oncogenes and tumor suppressor genes is involved in the development of ACC. However, the precise mechanism responsible for its oncogenesis is not completely understood -. Therefore, it is warranted to study the molecular mechanism of salivary gland ACC to gain insight into its diagnosis, prognosis, and treatment.
As a widely accepted tumor suppressor gene, RUNX3 (human runt-related transcription factor 3) functions in major physiological and pathological processes. Many reports have shown essential behavior of RUNX3 in a variety of cancers . Our previous study presented the expression of RUNX3 in normal salivary glands and salivary ACC. Moreover, the expression of RUNX3 is obviously correlated with histopathological growth pattern, T stage, distant metastasis, and patient’s survival. These results suggested that the low level of RUNX3 in salivary gland ACC might play a key role in tumor progression and have prognostic value in ACC . Subsequently, we found that the RUNX3 mislocalization was related to the progression of the ACC by laser scanning confocal microscope .
Recent studies have demonstrated that Pim-1, which acted as an oncogene, could phosphorylate RUNX3 and alter its subcellular localization ,. As belongs to the Ser/Thr kinase family, Pim kinases can phosphorylate a large range of cellular substrates to exert their physiological activities, which are involved in cell differentiation, cell proliferation, cell cycle and apoptosis. Pim-1 plays a pivotal role in tumorigenesis and overexpression of it has been implicated in several cancers ,. Collectively, these reports suggest that Pim-1’s function is important in the progression of cancer.
However, the oncogenic role of Pim-1 in ACC has not yet been examined. As an effective tool to achieve gene silence, small interfering RNAs (siRNAs) were used in lots of researches to clarify the gene function -. In this study, using siRNA transfection in vitro, we aim to clarify the gene function of Pim-1 in ACC through the detection of cell proliferation, cell cycle, cell apoptosis and cell invasion. Meanwhile, a Pim-1 inhibitor (SGI-1776) was used to confirm the affection of Pim-1 on the cell proliferation ability. The RUNX3, Cyclin D1 and CDK4 (cyclin-dependent kinase 4) expression after Pim-1 siRNA transfection was investigated as well. Furthermore, the relationship between Pim-1 and RUNX3 was deduced in the ACC tissues. The correlation of Pim-1/RUNX3 and clinical parameters were analyzed. This study provided new data of Pim-1 activity in ACC and is suggestive that Pim-1 has potential to become a tumor marker as well as a therapy target of cancer.
Materials and methods
Fifty-four patients with histopathologically proven salivary gland adenoid cystic carcinoma (ACC) in Zhejiang Cancer Hospital between July 2006 and July 2010 were included for this study. The study was approved by the Ethics Committee of Zhejiang Cancer Hospital, and patients have signed informed consent.
Cell culture and siRNA transfection
SACC-83 and SACC-LM were kind gifts from Prof. Li Shengling (Peking University School of Stomatology). SACC-83 is derived from human ACC tissue and SACC-LM is a lung metastasis cell line of SACC-83 ,. It is considered that SACC-LM is more malignant then SACC-83. Both cell lines were cultured in RPMI-1640 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (HyClone Laboratories, Logan, USA) in a humidified atmosphere of 5% CO2 at 37°C. Pim-1 siRNA and control siRNA were obtained from Santa Cruz Company (CA, USA). SACC-83 and SACC-LM cells were seeded in a 96-well culture plate with a density of 5 × 103 cells/well or in a 6-well culture plate with a density of 1 × 106 cells/well. After 24 h, siRNAs (0.1 μmol/L) were transfected with Oligofectamine TM Reagent (Invitrogen, Carlsbad, CA, USA) into the cells according to manufacturer's protocol.
SGI-1776 (Selleckchem, Houston, TX, USA) was dissolved in DMSO to obtain a stock solution at 10 mmol/L and stored at −80°C. After SACC-83 and SACC-LM were cultured, SGI-1776 was added to the culture system to achieve the final doses of 0–10 μmol/L. The highest concentration of DMSO in the culture was 0.1% and we tested that it had no effect on the cells viability.
Cell proliferation assay
According to manufacturer's instructions, Cell Counting Kit-8 (CCK-8) was used to determine the cell proliferation (Dojindo Biotechnology, China). Briefly, SACC-83 and SACC-LM cells were seeded in a 96-well cell plate for 24 h, and then transfected with Pim-1 siRNA/control siRNA, for 48 and 72 h and exposed to SGI-1776 for 24, 48 and 72 h respectively. Then the medium were discarded and replaced with 100 μl of fresh medium containing 10% CCK-8. After cells were incubated at 37°C for 4 h, the absorbance was detected at 450 nm on a microplate reader.
Colony formation assay
After transfected with Pim-1 siRNA and control siRNA for 72 h, SACC-83 and SACC-LM cells were washed with PBS (Phosphate Buffer Solution) and trypsined. Then the cells were seeded into a 12-well plate with a density of 500 cells/ well. After 7 days of incubation, colonies were stained by 0.5% crystal violet (Sigma-Aldrich, St. Louis, MO, USA) and counted directly.
Evaluation of live cell undergoing apoptosis
In this study, Annexin V-FITC and propidium iodide (PI) (BD Biosciences, USA) were used to distinguish intact, dead and apoptotic cells by using the flow cytometric method. SACC-83 and SACC-LM cells were harvested and washed with cold PBS after transfected with Pim-1 siRNA and control siRNA for 72 h. Subsequently, the cells were resuspended in 100 μL binding buffer. 5 μL Annexin V-FITC and 1 μL PI were added to the cell suspension and incubated in darkness at room temperature for 15 min. Thereafter, 400 μl binding buffer was added to each sample and then the cells were analyzed by using the flow cytometer (BDLSR, Becton Dickinson, USA).
Cell cycle detection
Cell cycle assay was performed using the Cycle Test Plus™ DNA Reagent Kit (340242, Becton Dickinson, USA) following the manufacturer’s instructions. SACC-83 and SACC-LM cells were harvested and washed with cold PBS after transfected with Pim-1 siRNA and control siRNA for 72 h. Then, the cells were fixed in pre-cooled 70% ethanol for 24 h at 4°C. After that, the cells were dyed with PI and detected by flow cytometry (BDLSR, Becton Dickinson, USA) to evaluate the cell cycle distribution.
Transwell chamber invasive assay
After transfected with Pim-1 siRNA and control siRNA for 72 h, SACC-83 and SACC-LM cells were obtained and plated at 1.0 × 105 cells/well in 0.5 mL of serum-free medium in 24-well matrigel-coated transwell units with polycarbonate filters (8 μm pore size; Costar Inc., Milpitas, CA, USA). The outer chambers were filled with 0.5 mL of RPMI 1640 medium supplemented with 10% FBS. After incubated for 24 h, the cells were fixed in methanol and stained with 2% crystal violet. The top surface of the membrane was gently removed and the invading cells were counted in five randomly selected fields under a microscope.
Assessment of mitochondrial membrane potential
The mitochondrial membrane potential (MMP), which is recognized as a typical marker of early apoptosis was measured by the fluorescent probe JC-1 (MultiSciences Company, Hangzhou, China) in this study. As a cationic and lipophilic dye, JC-1 presents a fluorescence emission shift from green (525 nm) to red (590 nm) to indicate the potential-dependent accumulation in mitochondria. In healthy and normal cells with low membrane potentials, JC-1 appears as a monomer and produces a green fluorescence. At high membrane potentials, J aggregates is induced by JC-1 and the red fluorescence was emerged. In accordance with the manufacturer's protocol, after SACC-83 and SACC-LM cells were transfected by the Pim-1 siRNA for 72 h, the cells were harvested, centrifuged and re-suspended in 1 ml staining buffer. After stained with 1ul JC-1 staining solutions, the cells were incubated at 37°C in the dark for 30 min. Subsequently, the cells were washed twice with warm PBS buffer and re-suspended in 0.5 ml PBS buffer. Flow cytometry was performed to examine the red/green fluorescent signals.
Quantitative real-time reverse transcription-PCR
SACC-83 and SACC-LM cells were transfected with Pim-1 siRNA or control siRNA for 72 h. Total RNA was extracted by using Trizol (Invitrogen, Carlsbad, CA, USA) and reverse-transcription was done with PrimeScipt™ RT reagent Kit (TAKARA BIO INC., Otsu, Shiga, Japan) according to manufacturer’s instructions. Quantitative real-time PCR (qRT-PCR) was performed on an ABI Prism 7500 instrument (Applied Biosystems, Foster city, CA, USA) by using the SYBR Premix Ex Taq (TAKARA BIO INC., Dalian, China). Pim-1 and GAPDH mRNA levels were measured by their specific primers:
GAPDH (F: 5'-GAAGGTGAAGGTCGGAGTC-3';
R: 5'- GAAGATGGTGATGGGATTTC-3')
After PCR amplification, the dissociation of SYBR Green-labeled cDNA was carried out to affirm that there were no nonspecific PCR products. 2-∆∆Ct method was performed to analyze the relative quantification of Pim-1 expression.
Western blot analysis
SACC-83 and SACC-LM cells were trypsinized and washed with cold PBS after transfected with Pim-1 siRNA or control siRNA for 72 h. Then cell pellet was lysed with RIPA Lysis Buffer (Beyotime, Nanjing, China). ACC tissue samples were ground with the Tissue Lyser-II (Qiagen, Germany) and lysed with RIPA Lysis Buffer. Protein concentrations were determined by the BCA Protein Assay Kit (Beyotime, Nanjing, China). All samples were stored at −70°C prior to electrophoresis. Each sample containing 50 μg of protein were subjected to 12% SDS-PAGE and separated by electrophoresis. Then the proteins were transferred electrophoretically from the gel to nitrocellulose membrane (Immobilon-PSQ Transfer Membrane, Millipore Corporation, Bedford, U.S.A.). To prevent non-specific binding of reagents, the membranes were blocked in TBS buffer (50 mM Tris-Cl, 150 mM NaCl, pH 7.6) containing 5% non-fat dry milk at room temperature for 3 h. Then blotted with primary antibodies of Pim-1 (Novus biologicals, Cambridge, UK) (1:1000 dilution), RUNX3 (Abgent, San Diego, CA, USA) (1:100 dilution), Cyclin D1 (Proteintech, Whhan, China) (1:1000 dilution), CDK4 (Proteintech, Whhan, China) (1:1000 dilution), β-actin (Abcam, New Territories, HK) (1:200 dilution) and GAPDH (Huabio Technology, Hangzhou, China) (1:2000 dilution) at 4°C overnight and developed with peroxidase-labeled secondary antibodies (Huabio Technology, Hangzhou, China). After that, the membranes were extensive washed in TBST and exposed to 2 mL ECL chemiluminescence reagent. The images were captured and analyzed on the Bio-Rad GelDoc XR.
Section (4 μm) of paraffin-embedded tissues were cut, mounted on glass slides (MS-coated glass, Mats-unami, Oaska, Japan), and dried overnight at 37°C. After deparaffinization, antigen retrieval in 0.01 M citrate buffer, and inactivation of endogenous peroxidase activity in 3% H2O2/methanol, the slides were incubated with antibody for Pim-1 (Novus biologicals, Cambridge, UK) (1:200 dilution) and RUNX3 (Abcam, New Territories, HK) (1:200 dilution) at 4°C overnight. The immunoreactivity was visualized using a streptavidin–biotin peroxidase staining kit (Histofine Simple Stain Max PO Multi, Nichirei, Tokyo, Japan) and DAB solution (Simple Stain DAB, Nichirei). The results were presented as percentage of nucleus staining positive cells and the total cells. The scores of staining results were given as negative and positive. The criterion was consulted our previous study .
All data were analyzed using SPSS 10.0 software. Results from in vitro experiments were expressed as mean ± standard deviations and statistically analyzed by the one-way analysis variance (ANOVA). Associations between Pim-1, RUNX3 levels and clinicopathologic parameters were analyzed using the X2 test or the Fisher exact test. Survival analysis was carried out by the Kaplan–Meier method and significant differences were assessed by means of the log-rank test. P values < 0.05 were considered to be statistical significance.
Effect of the Pim-1 siRNA on the Pim-1 level
Effect of Pim-1 RNAi on cell proliferation in SACC-83 and SACC-LM
Effect of SGI-1776 on cell proliferation in SACC-83 and SACC-LM
Effect of the Pim-1 siRNA on the apoptosis
Effect of the Pim-1 siRNA on cells cycle
Effect of the Pim-1 siRNA on cell invasion
Effect of the Pim-1 siRNA on the mitochondrial function
RUNX3, Cyclin D1 and CDK4 expression of SACC cells after Pim-1 siRNA transfection
Correlation between Pim-1 and RUNX3 protein levels in ACC tissues
Relationship between the level of Pim-1 and RUNX3
Patients (total = 54)
Correlation between the level of Pim-1 and RUNX3 and the clinical characteristics in ACC tissues
Relationship between the Pim-1, RUNX3 level and the clinical characteristics
Patients (total = 54)
Lymph node involvement
Increasing evidence shows the important role of Pim-1 in many cancers. Various investigations have linked Pim-1 to aggressive malignant behavior and poor clinical outcome in many cancer cells, including gastric cancer , prostate cancer , esophageal squamous cell carcinoma , breast cancer , lung cancer , colon cancer , and hematological cancer . However, few studies has concern the implications of Pim-1 in salivary ACC.
In this study, SACC-83 and SACC-LM cell lines were used to evaluate the function of Pim-1 in salivary ACC. After Pim-1 siRNA transfection, the mRNA and protein levels of Pim-1 were significantly decreased in both cell lines, suggesting that Pim-1 siRNA inhibits endogenous Pim-1 expression. CCK-8 assay and colony formation results revealed that down-regulation of Pim-1 could restrain the cell viability of SACC-83 and SACC-LM. Meanwhile, we use SGI-1776, which was confirmed as a novel inhibitor of the PIM kinases to test the function of Pim-1. SGI-1776 is an imidazo [1,2-b] pyridazine compound and have been proved to effectively reduced Pim-1 activity in several researches -. Our results displayed that SGI-1776 could evidently induced the SACC cells growth inhibition. It could be deduced from these results that Pim-1 is important for cell proliferation in ACC cells.
To disarrange cell cycle progression is one of the central features of malignant cancer cells. In the present study, it was found that G0-G1 phase cells were increased while G2-M phase and S phase cells were decreased after Pim-1 siRNA transfection. Furthermore, we observed the down-regulation of Cyclin D1 and CDK4 protein expression. Cyclin D1 and CDK4 work together to form the complex and to promote G1 phase progression and regulate the cell cycle G1/S transition . Those data demonstrates that the G0/G1 cell cycle arrest was induced by knocking down Pim-1 and probably mediated by Cyclin D1 and CDK4. Annexin V-FITC/PI and transwell assay results showed that the apoptosis rates were dramatically ascended and the invasion ability was significantly reduced after Pim-1 siRNA transfection in both cell lines. Meanwhile, the mitochondrial dysfunction indicated by membrane potential decrease after siRNA transfection in both cells was investigated. These findings reinstates the oncogenic function of Pim-1 in ACC cell lines, indicating the important role of Pim-1 in tumorigenesis of ACC.
Meanwhile, the transwell migration results show that Pim-1 siRNA could dramatically reduce the invasion capacity of SACC cells. The invasion inhibition effect by the Pim-1 siRNA was stronger in the SACC-83 cells than in the SACC-LM cells. The immunohistochemical results show that Pim-1 level is significantly associated with nerve invasion in ACC patients. The findings suggest Pim-1 expression may be a critical marker for ACC invasion.
In 2006, Aho et al. found that the C-terminal part of human RUNX3 associates with Pim-1 by using the yeast two-hybrid system . Subsequently, Kim et al. demonstrated that Pim-1 phosphorylates four Ser/Thr residues within the Runt domain and stabilizes RUNX3 protein . In SACC-83, we observed an increase of RUNX3 protein level after Pim-1 transfection. In ACC tissues, there was a significant reverse correlation between the Pim-1 and RUNX3 expression by IHC evaluation.
Moreover, we investigated the Pim-1 and RUNX3 in ACC tissues. The IHC results show that both Pim-1 and RUNX3 levels were significantly associated with T stage and nerve invasion. Patients with advanced T stage and nerve invasion had a higher Pim-1 and lower RUNX3 level. High expression of Pim-1 and low expression of RUNX3 were associated with aggressive tumor behavior. This evidence suggests an importance of the interaction between Pim-1 and RUNX3 in ACC. As to other clinical features such as gender, age, tumor location, histological grade type, lymph node involvement or distant metastasis, we did not found significant associations between the Pim-1/RUNX3 and them, which might owing to the limitation of the cancer quantity and need to be further studied.
Survival analysis indicated that Pim-1 level had a weak association with overall survival of the ACC patients (p = 0.091). Patients with lower Pim-1 level had a better outcome than that with higher Pim-1 level. Choi et al. found that Pim-1 expression might be used as a possible prognostic factor in laryngeal squamous cell carcinoma . Peng et al. presented that expression of Pim-1 in tumors, tumor stroma, and tumor-adjacent mucosa could indicate the prognosis of colon cancer patients . Moreover, Jin et al. confirmed that overexpression of Pim-1 associated with poor prognosis of non-small cell lung cancer . Our findings corroborate the findings of these studies, yet more studies will be needed to define mechanisms of Pim-1 expression and function in ACC.
The present study demonstrates that Pim-1 is important in cell proliferation, apoptosis, cell cycle, and invasion in both SACC-83 and SACC-LM cell lines. Pim-1 and RUNX3 levels were negative relevant and significantly associated with T-stage and nerve invasion in the ACC tissues. This study approves the oncogenic role of Pim-1 in ACC. The findings also suggest that Pim-1 may serve as a neoteric therapeutic target and potential prognostic marker for ACC cancer.
This research was supported by National Natural Science Foundation of China (No: 81202127), Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents (No .2008-134), and Zhejiang Medical and Health Science and Technology Plan (No. 2010KYA033, No. 2012KYA031, No. 2013KYB033).
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