- Open Access
6-Nitro-2-(3-hydroxypropyl)-1H-benz[de]isoquinoline-1,3-dione, a potent antitumor agent, induces cell cycle arrest and apoptosis
Journal of Experimental & Clinical Cancer Research volume 29, Article number: 175 (2010)
Anticancer activities of several substituted naphthalimides (1H-benz[de]isoquinoline-1,3-diones) are well documented. Some of them have undergone Phase I-II clinical trials. Presently a series of ten N-(hydroxyalkyl) naphthalimides (compounds 1a-j) were evaluated as antitumor agents.
Compounds 1a-j were initially screened in MOLT-4, HL-60 and U-937 human tumor cell lines and results were compared with established clinical drugs. Cytotoxicities of compounds 1d and 1i were further evaluated in a battery of human tumor cell lines and in normal human peripheral blood mononuclear cells. Cell cycle analysis of compound 1i treated MOLT-4 cells was studied by flow cytometry. Its apoptosis inducing effect was carried out in MOLT-4 and HL-60 cells by flow cytometry using annexin V-FITC/PI double staining method. The activities of caspase-3 and caspase-6 in MOLT-4 cells following incubation with compound 1i were measured at different time intervals. Morphology of the MOLT-4 cells after treatment with 1i was examined under light microscope and transmission electron microscope. 3H-Thymidine and 3H-uridine incorporation in S-180 cells in vitro following treatment with 8 μM concentration of compounds 1d and 1i were studied.
6-Nitro-2-(3-hydroxypropyl)-1H-benz[de]isoquinoline-1,3-dione (compound 1i), has exhibited maximum activity as it induced significant cytotoxicity in 8 out of 13 cell lines employed. Interestingly it did not show any cytotoxicity against human PBMC (IC50 value 273 μM). Cell cycle analysis of compound 1i treated MOLT-4 cells demonstrated rise in sub-G1 fraction and concomitant accumulation of cells in S and G2/M phases, indicating up-regulation of apoptosis along with mitotic arrest and/or delay in exit of daughter cells from mitotic cycle respectively. Its apoptosis inducing effect was confirmed in flow cytometric study in MOLT-4 and the action was mediated by activation of both caspase 3 and 6. Light and transmission electron microscopic studies corroborated its apoptosis inducing efficacy at a concentration of 10 μM in MOLT-4 cells. Its apoptosis induction was also observed in HL-60 cells to an extent much greater than well known apoptosis inducing agents as camptothecin and cis-platin at 10 μM concentration each. It significantly inhibited DNA and RNA synthesis in S-180.
In essence, compound 1i showed potential as an antitumor agent.
Development of an anticancer compound is always a fascinating challenge in the field of cancer chemotherapy. Research is ongoing globally to identify new leads. The anticancer activities of several substituted naphthalimides (1H-benz[de]isoquinoline-1,3-diones) are well documented [1, 2]. For example, substituted naphthalimides containing N-(2,2-dimethylaminoethyl) chain best represented by Mitonafide (5-nitro group in the aromatic ring) and Amonafide (5-amino group in the aromatic ring) have been shown to possess significant anticancer activities. Both Mitonafide [3, 4] and Amonafide [5, 6] have undergone Phase I-II clinical trials with limited success. We have recently reported appreciable antitumor activity of some new compounds belonging to N-(2-chloroethyl)- and N-(3-chloropropyl) naphthalimides . From the literature search, it was found that there was no report, to our knowledge, that describes the anticancer potential of known N-(2-hydroxyethyl) and N-(3-hydroxypropyl) naphthalimides (compounds 1a-j). Hence we have undertaken the present study of evaluating their potency. In this report we have documented the findings that shows that 6-nitro-2-(3-hydroxypropyl)-1H-benz[de]isoquinoline-1,3-dione (compound 1i) is the most active member in the series.
Materials and methods
Chemicals and drugs
A total number of ten substituted 2-(2-hydroxyethyl)- and 2-(3-hydroxypropyl)-1H-benz[de]isoquinoline-1,3-diones (compounds 1a-j) (Figure 1) were prepared following established procedure. Out of these ten compounds, test compound 1i was most extensively investigated. Mitonafide was received earlier as a gift from Prof. M.F. Brana, University of San Pablo-CEU, Madrid, Spain. Anticancer drugs, propidium iodide and annexin V-FITC detection kit (A2214) were procured from Sigma-Aldrich Corporation, St. Louis, MO, USA.
Culture of human tumor cell lines
The following human tumor cell lines namely Leukemia: acute lymphoblastic MOLT-4, promyelocytic HL-60; Lymphoma: histiocytic U-937; Breast: MCF-7; Neuroblastoma: IMR-32, SK-N-SH; Colon: 502713, COLO-205, HCT-15, SW-620; Liver: Hep-2; Prostate: DU-145, PC-3 and Lung: A549 obtained either from National Centre of Cell Science (NCCS), Pune, India or National Cancer Institute, Fredrick, MD, USA were used. Cell lines were grown in tissue culture flasks in RPMI-1640 medium with 2 mM glutamine (Invitrogen Corporation, USA) containing 1% antibiotics (100 units penicillin/ml and 100 μg streptomycin/ml, Cambrex Bioscience Inc., USA), pH 7.4, sterilized by filtration and supplemented with 10% heat-inactivated fetal bovine serum (FBS, Invitrogen Corporation, USA) at 37°C in an atmosphere of 5% CO2/95% relative humidity in a CO2 incubator and routinely sub-cultured. Trypsin (0.02%) was used for dislodging adherent type cells.
In vitro screening in human tumor cell lines
All the test compounds 1a-j were initially screened against U-937 and HL-60 cell lines by MTT assay as per standard procedure . Compounds 1d and 1i were also screened in MOLT-4 (Table 1). Drug stock solutions (20 mg/ml) were prepared in cell culture DMSO. These were serially diluted with complete growth medium stated above to obtain different drug concentrations [final DMSO concentration was 0.5% highest to 0.001% lowest]. Cells were seeded at 1 × 104 (U-937), 2 × 104 (HL-60) or 1 × 105 (MOLT-4) per well in 96-well cell culture plates and incubated with respective drug solutions of different concentrations for 96 hr and processed. All vehicle controls contained same concentration of DMSO. The plate was read in a microplate reader at 540 nm. Curvefit software was used to calculate the IC50 values. IC50 value < 10 μM is considered as active as per National Cancer Institute (NCI), USA, protocol.
Cytotoxicities of test compounds 1d and 1i were further evaluated against 11 other human tumor cell lines by SRB assay method  as stated in Table 2. Growth inhibition value 50% or more at 1 × 10-5M is considered as active. Established anticancer drugs such as doxorubicin, 5-FU, cis-platin, BCNU, hydroxyurea, paclitaxel and mitomycin C were used in parallel for comparison as indicated in the respective Table 1 and 2.
Effect on PBMC
PBMC was isolated from heparinized venous blood obtained from healthy human volunteer by Ficoll-Paque (Histopaque 1077, Sigma-Aldrich Corporation, St. Louis, MO, USA.) density gradient centrifugation as per standard procedure . PBMC (1 × 105 cells/well) were cultured in complete RPMI-1640 media as usual and incubated with compounds 1d and 1i for 48 hr followed by MTT assay. IC50 values were calculated using Curvefit software.
Analysis of cell cycle
The effect of compound 1i on different phases of cell cycle of MOLT-4 was explored by flow cytometry . In brief, 1 × 106 MOLT-4 cells were incubated with compound 1i (10.0 and 16.7 μM) for 24 hr and camptothecin (5 μM) for 3 hr. The cells were next washed twice with ice-cold phosphate buffered saline (PBS), harvested, fixed with ice-cold PBS in 70% ethanol, and stored at -20°C for 30 min. After fixation, the cells were incubated with RNase A (Sigma-Aldrich Corporation, St. Louis, MO, USA, 0.1 mg/ml) at 37°C for 30 min, stained with propidium iodide (Sigma-Aldrich Corporation, St. Louis, MO, USA, 50 μg/ml) for 30 min on ice in dark and analyzed for DNA content using BD-LSR Flow cytometer (Becton Dickinson, USA). Data were collected in list mode on 10,000 events and analyzed using Mod Fit 2.0 software (Figure 2).
Assessment of apoptosis
Annexin V-FITC/PI double staining method was followed  for the assay in MOLT-4 cells (1 × 106/well, 6-well plate) after incubation of the cells with 10.0 and 16.7 μM of compound 1i and 5 μM of camptothecin for 6 hr at 37°C (Figure 3). Similar assay was conducted in HL-60 by using another apoptosis detection kit (BD Biosciences Pharmingen, San Diego, USA). For this, HL-60 cells (5 × 105/well) were treated for 24 hr with compounds 1i, camptothecin and cis-platin (10 μM concentration each). Cells were processed and stained with Annexin V-FITC/PI according to the manufacturer's instructions and analyzed on a FACScan flow cytometer (Becton Dickinson, USA) using Cell Quest software at two wavelengths 515 and 639 nm. Vehicle (DMSO) treated unstained and stained [annexin V-FITC/PI] cells were used as controls (Figure 4).
Measurement of caspase-3/6 activities
The activities of caspase-3 and caspase-6 in MOLT-4 cells (2 × 106/ml) following incubation with compound 1i (3.3 - 16.7 μM) and camptothecin (5 μM) for variable periods were measured by using respective colorimetric assay kit (R&D Systems, USA). Blank cell lysate control was also included. Enzyme-catalyzed release of pNA was monitored using a microplate reader at 405 nm (Figure 5A and 5B).
Cell morphological and ultra structural assessment
MOLT-4 cells were incubated with compound 1i (10 μM) in DMSO for different time periods. Control cells received DMSO only (< 0.5%). Treated and control cells were washed in PBS, centrifuged at 1500 rpm for 10 min. Pellets were divided into 1 mm3 pieces and fixed immediately in 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) for 2 hr at 4°C, post-fixed with 1% OsO4 in the same buffer for 2 hr, dehydrated with acetone, cleared in propylene oxide and embedded in Epon-812 . Semithin (1 μm) sections were cut, stained with toluidine blue and morphology of treated cells was observed  at different times under light microscope [Olympus, Japan]. Photomicrographs were taken with Olympus Digital Camera (C4000) (Figure 6). Ultrathin sections of silver color (60-90 nm) were cut on a LKB ultramicrotome IV, mounted on copper grids and stained with uranyl acetate and lead citrate. The sections were viewed and photographed in a JEOL-100CXII electron microscope at 60 kV (Figure 7).
3H-Thymidine and 3H-Uridine incorporation in S-180 cells in vitro
S-180 tumor cells maintained in vivo in Swiss albino mice were used for incorporation of 3H-thymidine and 3H-uridine (specific activity 1.0 mCi/ml each, obtained from Board of Radiation and Isotope Technology, Mumbai, India) following treatment with 8 μM concentration of compounds 1d and 1i as described earlier . Mitonafide at the same concentration was used for comparison.
Values were recorded as the mean ± S.E.M. (standard error mean) of three experiments. Experimental results were analyzed by Student's t-test. P < 0.05 was considered as the level of significance for values obtained for treated groups compared with control group.
In vitro screening of compounds 1a-j against U-937 and HL-60 revealed that compounds 1a-c, 1e-1h and 1j did not show appreciable activity as their IC50 values were above 10 μM. Compounds 1d and 1i having IC50 values in the range of 0.7 and 6.0 μM in U-937, HL-60 and MOLT-4 were found to be cytotoxic (Table 1). The IC50 values of compounds 1d and 1i were much less than that of doxorubicin, 5-FU, cis-platin, BCNU and hydroxyurea used as standards (Table 1) suggesting greater antitumor properties in compounds 1d and 1i. In view of this, compounds 1d and 1i were selected for further screening in a battery of human tumor cell lines. The results summarized in Table 2 revealed that compound 1d has elicited significant growth inhibition in two (IMR-32 and COLO-205) out of six cell lines used while compound 1i elicited significant growth inhibition in five (SK-N-SH; 502713, SW-620, DU-145 and PC-3) out of ten cell lines tested. It appears that compound 1i is the most active member.
In vitro toxicity screening in PBMC
Compounds 1d and 1i showed high IC50 values of 698 and 273 μM respectively against human PBMC in vitro suggesting that these compounds were devoid of significant cytotoxicity against normal cells.
Effect on cell cycle
MOLT-4 cells exposed to 10.0 and 16.7 μM of compound 1i for 24 hr exhibited increase in sub-G1 fraction which may comprise of both apoptotic cells and cell debris implying up-regulation of cell death machinery. The effect was much more for the higher concentration of the compound. For instance, the sub-G1 fractions of control and camptothecin-treated cells were 0.68% and 11.92% respectively whereas the same were 4.69% and 21.02% for compound 1i at the low and high concentrations (Figure 2). This might indicate a dose dependant increase in apoptosis of MOLT-4 cells inflicted by compound 1i. The cell cycle analysis also showed accumulation of treated cells in S and G2/M phases. Increase in S phase fraction could be due to stimulation of DNA synthesis or delay in movement of cells from S to G2/M phase. Concomitant rise in G2/M fraction indicates delay in exit of daughter cells from the mitotic cycle. Therefore the findings suggest delayed turnover of cells leading to reduction of tumor cell number.
Analysis of apoptosis in MOLT-4 and HL-60 cells by Annexin V-FITC/PI double staining method
MOLT-4 and HL-60 control and treated cells were stained with annexin V-FITC/PI and gated into LR (Lower Right) and UR (Upper Right) quadrants. Cells in LR and UR were considered as early apoptotic (annexin+/PI-) and late apoptotic (annexin+/PI+) respectively. Extent of apoptosis was expressed as the sum total of the percentages in LR and UR quadrants. Cells in LL (Lower Left) and UL (Upper Left) quadrants were considered live and necrotic respectively. Apoptosis induced by compound 1i was compared with that of camptothecin (Figure 3) and camptothecin and cis-platin used as standards (Figure 4). Apoptosis recorded in untreated control MOLT-4 and HL-60 cells were 3.61% and 2.54% respectively.
In MOLT-4, total apoptosis exhibited by camptothecin at 5 mM concentration was 8.89%. In contrast compound 1i at 10.0 and 16.7 mM concentrations was effective in inducing 27.54% and 30.86% apoptosis respectively. The necrotic cell populations for compound 1i at these doses were 5.15% and 4.80% respectively (Figure 3).
In HL-60, compound 1i induced 98.62% apoptosis at a dose of 10 μM (LR 3.49%, UR 95.13%). This is in contrast to 15.82% and 7.51% apoptosis respectively induced by camptothecin and cisplatin at the same dose. Thus compound 1i was more effective than standards in inducing apoptosis in HL-60 (Figure 4).
Activation of caspases
Treatment of MOLT-4 cells with compound 1i was associated with marked increase in caspase-3 as well as caspase-6 activities that confirm the apoptotic mode of cell death. Up-regulation of caspase-3 by compound 1i was maximum at 5.0 μM concentration at 12 hr post-treatment (Figure 5a) while caspase-6 activity was highest also at 5.0 μM concentration at 24 hr post-treatment (Figure 5b). Similar activations were produced by camptothecin at 5.0 μM concentration (Figure 5a-b).
Cell morphological and ultra structural assessment
The morphology of MOLT-4 cells treated with compound 1i at 5 and 10 μM was monitored by light microscopy at different time points. The number of apoptotic cells increased with higher concentration of the compound and longer incubation period. Figure 6b represents the characteristic morphology of apoptotic cells following 36 hr of incubation at 10 μM concentration. Marginalization of chromatin material accompanied by cell shrinkage, nuclear condensation/fragmentation and formation of cytoplasmic vacuoles, considered as hallmark of apoptosis, were clearly visible. Control cells showed large sized nuclei having nucleoli (Figure 6a).
In transmission electron microscopy, MOLT-4 control cells (Figure 7a-b) exhibited a high nucleocytoplasmic ratio and the nucleus had a finely dispersed chromatin with nuclear pores. The nucleoli were clearly visible in most of the cells. The mitochondria with cristae (MC) in various size and shape (oval and elongated), rough endoplasmic reticulum and ribosomes were seen. MOLT-4 cells treated with 10 μM of compound 1i for 36 h revealed damaged mitochondrial cristae and highly reduced rough endoplasmic reticulum suggesting apoptosis (Figure 7c-f). No inflammatory changes in nuclei and cytoplasm coupled with absence of breakage in plasma membrane ruled out the possibility of necrotic events. Vacuolization was also seen in treated cells. Literature survey also revealed similar observations [16, 17].
Inhibition of DNA/RNA synthesis in S-180 tumor cells in vitro
Since compound 1d and 1i have structural similarity with mitonafide, studies were conducted to ascertain whether drug-induced tumor growth inhibition was also due to the inhibitory effect of these compounds on nucleic acid synthesis. Accordingly 3H-thymidine and 3H-uridine incorporation by S-180 cells collected from untreated tumor bearing mice was measured after treating the tumor cells in vitro. The untreated S-180 cells demonstrated an almost linear pattern of 3H-thymidine and 3H-uridine incorporation over a period of 60 min. Exposure of tumor cells to test compounds at the concentration of 8 μM resulted in gradual and marked inhibition of 3H-thymidine and 3H-uridine incorporation comparable to that of mitonafide at the same concentration (8 μM). After 1 hr of incubation with compound 1d and 1i3H-thymidine incorporation was declined by 96% and 95% respectively against 95% reduction by mitonafide exposure. Thus the compounds showed remarkable inhibitory effect on DNA synthesis. Inhibition of RNA synthesis, in contrast was less spectacular as inhibition of 3H-uridine was 92%, 94% and 89% for mitonafide, compound 1d and 1i respectively (Figure 8).
The nature and position of a substituent in a molecule are known to play important roles in deciding its antitumor property. The present study has shown that out of the five different substituents (R = H, 6-Br, 6-Cl, 6-NO2, 5-NO2) present in the aromatic ring portion of substituted N-(hydroxyalkyl)naphthalimide moiety, the 6-NO2 substituent is crucial in exercising the antitumor activity. This is in agreement with our earlier finding in other (chloroalkyl) naphthalimide compounds wherein we found 6-nitro-2-(3-chloropropyl) naphthalimide as the most active antitumor agent in that series .
Compound 1i that showed most pronounced antitumor activity interfered with S and G2/M phases of cell cycle of MOLT-4 cells. As a preparatory step towards cell division, a cell duplicates its DNA in S phase of cell cycle. Thus, interference of S phase by compound 1i as observed in flow cytometric measurements, suggests that it affects DNA duplication process of tumor cell before mitosis. This possibility was confirmed in S-180 cells in which compound 1i inhibited 3H-thymidine incorporation into DNA, implying suppression of DNA synthesis. Moreover, it inhibited 3H-uridine uptake, indicating concomitant inhibition of RNA synthesis. Taken together, the results suggest that inhibition of DNA and RNA might have played a role in mediating the antitumor effect of compound 1i.
Delay in exit from G2/M, the final phase of cell cycle, was another flow cytometric observation in compound 1i treated MOLT-4 cells. A situation like this develops when there is defect in DNA damage repair, spindle attachment with centromeres and polymerization of spindle microtubules . In view of these reports, it appears that the compound has adverse effect on the mitotic apparatus causing up-regulation of the spindle checkpoint control leading to delayed mitotic exit of daughter cells. It is known that vinca alkaloids  and paclitaxel  mediate their antitumor effects by interfering with spindle microtubules. Compound 1i may act in a similar fashion like them.
Induction of apoptosis or programmed cell death is a common mechanistic pathway of several antitumor agents . Compound 1i has exerted its antitumor action by this pathway as well. This is evident from sharp rise in sub-G1 fraction, light and electron microscopic studies showing morphological imprints of apoptosis and marked increase in caspase 3 and 6 in treated cells. Apoptosis is controlled by a diverse range of cell signals which may originate intracellularly via the mitochondria or extracellularly via death receptors on cell membranes. These two pathways of signals converge and form a common irreversible execution phase mediated by caspase 3 and 6. Whether the pro-apoptotic signal elicited by compound 1i followed the intrinsic (mitochondrial) or extrinsic (death receptor) pathway is not clearly understood. However, extensive damage of mitochondrial cristae in treated cells, as observed in ultrastructural study, favours mitochondrial pathway. Like the present finding, induction of apoptosis by many naphthalimides including amonafide and amonafide analogs has been reported [22, 23].
In essence, the present study demonstrated significant antitumor activity by compound 1i against murine S-180 tumor cells and a panel of human tumor cell lines in vitro and the effect was mediated by inhibition of cell proliferation and up-regulation of programmed cell death. Since the compound did not elicit any cytotoxicity against normal human PBMC, it holds promise for further development as a potential antitumor agent.
peripheral blood mononuclear cells
50% inhibitory concentration
Brana MF, Castellano JM, Roldan CM, Santos C, Vazquez C, Jimenez A: Synthesis and mode(s) of action of a new series of imide derivatives of 3-nitro-1,8-naphthalic acid. Cancer Chemother Pharmacol. 1980, 4: 61-66. 10.1007/BF00255461.
Brana MF, Castellano JM, Moran M, Perez de Vega MJ, Romerdahl CA, Qian XD, Bousquet P, Emling F, Schlick E, Keilhauer G: Bis-naphthalimides: a new class of antitumor agents. Anti-Cancer Drug Design. 1993, 8: 257-268.
Llombart M, Poveda A, Forner E, Martos CF, Gaspar C, Munoz M, Olmos T, Ruiz A, Soriano V, Benavides A, Martin M, Schlick E, Guillem V: Phase I study of mitonafide in solid tumors. Invest New Drugs. 1992, 10: 177-181. 10.1007/BF00877243.
Casado A, Rosell R, García-Gómez R, Díaz-Rubio E, Pérez-Manga G, Font A, Benavides A, Martín M: Phase II study of mitonafide in non-small cell lung cancer (NSCLC). Invest New Drugs. 1996, 14: 415-417. 10.1007/BF00180820.
Ratain MJ, Mick R, Berezin F, Janisch L, Schilsky RL, Vogelzang NJ, Lane LB: Phase I Study of Amonafide Dosing Based on Acetylator Phenotype. Cancer Res. 1993, 53: 2304-2308.
Leaf AN, Neuberg D, Schwartz EL, Wadler S, Ritch PS, Dutcher JP, Adams GL: An ECOG phase II study of amonafide in unresectable or recurrent carcinoma of the head and neck (PB390). Eastern Cooperative Oncology Group. Invest New Drugs. 1997, 15: 165-172. 10.1023/A:1005823703909.
Mukherjee A, Hazra S, Dutta S, Muthiah S, Mondhe DM, Sharma PR, Singh SK, Saxena AK, Qazi GN, Sanyal U: Antitumor efficacy and apoptotic activity of substituted chloroalkyl 1H-benz[de]isoquinoline-1,3-diones: a new class of potential antineoplastic agents. Invest New Drugs. 2010
Grayshan PH, Kadhim AM, Peters AT: Heterocyclic derivatives of naphthalene-1,8-dicarboxylic anhydride. Part III. (1) Benzo[k,l]thioxanthene-3,4-dicarboximides. J Heterocycl Chem. 1974, 11: 33-38. 10.1002/jhet.5570110107.
Skehan P, Storeng R, Scudiero D, Monks A, Mcmahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR: New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990, 82: 1107-1112. 10.1093/jnci/82.13.1107.
Monks A, Scudiero D, Skehan P, Shoemaker R, Paul K, Vistica D, Hose C, Langley J, Cronise P, Wolff AV, Goodrich MG, Campbell H, Mayo J, Boyd M: Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst. 1991, 83: 757-766. 10.1093/jnci/83.11.757.
Sharma MD, Ghosh R, Patra A, Hazra B: Synthesis and antiproliferative activity of some novel derivatives of diospyrin, a plant-derived naphthoquinonoid. Bioorg Med Chem. 2007, 15: 3672-3677. 10.1016/j.bmc.2007.03.050.
Yeruva L, Pierre KJ, Elegbede A, Wang RC, Carper SW: Perillyl alcohol and perillic acid induced cell cycle arrest and apoptosis in non-small cell cancer cells. Cancer Lett. 2007, 257: 216-222. 10.1016/j.canlet.2007.07.020.
Thornberry NA: Caspases: Key mediators of apoptosis. Chem Biol. 1998, 5: R97-103. 10.1016/S1074-5521(98)90615-9.
Reno F, Tontini A, Burattini S, Papa E, Falcieri E, Tarzia G: Mimosine induces apoptosis in the HL60 human tumor cell line. Apoptosis. 1999, 4: 469-477. 10.1023/A:1009608628076.
Mukherjee A, Dutta S, Sanyal U: Evaluation of Dimethoxydop-NU as a novel anti-tumor agent. J Exp Clin Cancer Res. 2007, 26: 489-497.
Ramirez CD, Catchpoole DR: Etoposide-induced apoptosis in lympho-blastoid leukemic MOLT-4 cells: Evidence that chromatin condensation, loss of phosphatidylserine asymmetry and apoptotic body formation can occur independently. Apoptosis. 2002, 7: 59-68. 10.1023/A:1013564928999.
Wyllie AH, Kerr JF, Currie AR: Cell death: the significance of apoptosis. Int Rev Cyt. 1980, 68: 251-306. 10.1016/S0074-7696(08)62312-8.
Reider CL, Schultz A, Cole R, Sluder G: Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle. J Cell Biol. 1994, 127: 1301-1310. 10.1083/jcb.127.5.1301.
Huang Y, Fang Y, Wu J, Dziadyk JM, Zhu X, Sui M, Fan W: Regulation of Vinca alkaloid-induced apoptosis by NF-κB/IκB pathway in human tumor cells. Mol Cancer Ther. 2004, 3: 271-277.
Wang TH, Wang HS, Soong YK: Paclitaxel-induced cell death: where the cell cycle and apoptosis come together. Cancer. 2000, 88: 2619-2628. 10.1002/1097-0142(20000601)88:11<2619::AID-CNCR26>3.0.CO;2-J.
Hickman JA: Apoptosis induced by anticancer drugs. Cancer Metastasis Rev. 1992, 11: 121-139. 10.1007/BF00048059.
Zhu H, Huang M, Yang F, Chen Y, Miao ZH, Qian XH, Xu YF, Qin YX, Luo HB, Shen X, Geng MY, Cai YJ, Ding J: R16, a novel amonafide analogue, induces apoptosis and G2-M arrest via poisoning topoisomerase II. Mol Cancer Ther. 2007, 6: 484-495. 10.1158/1535-7163.MCT-06-0584.
Tian ZY, Xie SQ, Du YW, Ma YF, Zhao J, Gao WY, Wang CJ: Synthesis, cytotoxicity and apoptosis of naphthalimide polyamine conjugates as antitumor agents. Eur J Med Chem. 2009, 44: 393-399. 10.1016/j.ejmech.2008.02.044.
We express our sincere thanks to the Council of Scientific and Industrial Research, New Delhi, India, for financial assistance [Grant Number: 01(1791)/02/EMR-II to U.S.], to Dr. Jaydip Biswas, Director, CNCI, for encouragement, to Dr. Manas Ranjan Ray, Head, Department of Experimental Hematology, CNCI, for helpful discussions and to Dr. Rathindranath Baral, Head, Department of Immunoregulation and Immunodiagnostics, CNCI, for flow cytometric experiments.
The authors declare that they have no competing interests.
US and AKS designed and co-coordinated the study at the respective Institutes [CNCI & IIIM]. AM and SD prepared the compounds & have carried out various biological experiments. MS carried out in vitro cytotoxicity screening in human tumor cell lines. DMM participated in the design of the study and performed cell cycle analysis. PRS performed cell morphological and ultra structural assessment. SKS carried out assessment of apoptosis and measurement of caspase-3/6 activities. AKS has helped to draft the manuscript. US has analyzed the data and prepared the manuscript. All authors have read and approved the final manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Mukherjee, A., Dutta, S., Shanmugavel, M. et al. 6-Nitro-2-(3-hydroxypropyl)-1H-benz[de]isoquinoline-1,3-dione, a potent antitumor agent, induces cell cycle arrest and apoptosis. J Exp Clin Cancer Res 29, 175 (2010). https://doi.org/10.1186/1756-9966-29-175
- Human Tumor Cell Line
- Human PBMC
- Double Staining Method
- Untreated Tumor Bear Mouse
- Spindle Checkpoint Control